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Branch production and fragmentation in the conidia of Pseudozyma prolifica Wiebe, Marilyn Gail
Abstract
The hyphomycete Pseudozyma prolifica Bandoni was grown in batch and continuous liquid cultures to determine the influence of growth rate and nutrition on conidium development. In batch culture, a period of elongation and branch formation, followed by fragmentation, was typically observed. The stage of branch formation was almost completely eliminated when the amino acids phenylalanine, glutamic acid, or asparagine were substituted for nitrate. Substituting citric acid for glucose had a similar effect. Branch formation was enhanced in sucrose + nitrate medium. In batch cultures, branched growth occurred at the start of the exponential growth phase. In continuous culture, the extent of branching was dependent on the specific growth rate. Maximal branching was observed at growth rates near the maximum. Growth by elongation and "bud" formation was predominant at low growth rates, as at the end of the log phase in batch cultures. The conidia were also examined using fluorescence and electron microscopy. Staining with wheat germ agglutinin conjugated to Fluorescein Isothiocyanate indicated that some intercalary growth occurs.
Item Metadata
Title |
Branch production and fragmentation in the conidia of Pseudozyma prolifica
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Creator | |
Publisher |
University of British Columbia
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Date Issued |
1986
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Description |
The hyphomycete Pseudozyma prolifica Bandoni was grown in batch and continuous liquid cultures to determine the influence of growth rate and nutrition on conidium development. In batch culture, a period of elongation and branch formation, followed by fragmentation, was typically observed. The stage of branch formation was almost completely eliminated when the amino acids phenylalanine, glutamic acid, or asparagine were substituted for nitrate. Substituting citric acid for glucose had a similar effect. Branch formation was enhanced in sucrose + nitrate medium.
In batch cultures, branched growth occurred at the start of the exponential growth phase. In continuous culture, the extent of branching was dependent on the specific growth rate. Maximal branching was observed at growth rates near the maximum. Growth by elongation and "bud" formation was predominant at low growth rates, as at the end of the log phase in batch cultures.
The conidia were also examined using fluorescence and electron microscopy. Staining with wheat germ agglutinin conjugated to Fluorescein Isothiocyanate indicated that some intercalary growth occurs.
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Genre | |
Type | |
Language |
eng
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Date Available |
2010-07-08
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Provider |
Vancouver : University of British Columbia Library
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Rights |
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
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DOI |
10.14288/1.0096885
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URI | |
Degree | |
Program | |
Affiliation | |
Degree Grantor |
University of British Columbia
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Campus | |
Scholarly Level |
Graduate
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Aggregated Source Repository |
DSpace
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Item Media
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Rights
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.