Branch production and fragmentation in the conidia of Pseudozyma prolifica
department or school: Science, Faculty of
department or school: Botany, Department of
data provider: DSpace
degree campus: UBCV
creator: Wiebe, Marilyn Gail
issued: 2010-07-08T03:52:38Z
issued: 1986
related degree: Master of Science - MSc
degree grantor: University of British Columbia
description: The hyphomycete Pseudozyma prolifica Bandoni was grown in batch and continuous liquid cultures to determine the influence of growth rate and nutrition on conidium development. In batch culture, a period of elongation and branch formation, followed by fragmentation, was typically observed. The stage of branch formation was almost completely eliminated when the amino acids phenylalanine, glutamic acid, or asparagine were substituted for nitrate. Substituting citric acid for glucose had a similar effect. Branch formation was enhanced in sucrose + nitrate medium. In batch cultures, branched growth occurred at the start of the exponential growth phase. In continuous culture, the extent of branching was dependent on the specific growth rate. Maximal branching was observed at growth rates near the maximum. Growth by elongation and "bud" formation was predominant at low growth rates, as at the end of the log phase in batch cultures. The conidia were also examined using fluorescence and electron microscopy. Staining with wheat germ agglutinin conjugated to Fluorescein Isothiocyanate indicated that some intercalary growth occurs.
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/26202
note: BRANCH PRODUCTION AND FRAGMENTATION IN THE CONIDIA OF PSEUDOZYMA PRQLD7ICA By MARILYN GAIL WIEBE B.Sc, The University o f Saskatchewan, 1984 A THESIS SUBMITTED IN PARTIAL FULiFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE in THE FACULTY OF GRADUATE STUDIES (Department o f Botany) We accept t h i s thesis as conforming -—teethe required standard THE UNIVERSITY OF BRITISH COLUMBIA August 1986 © Marilyn G a i l Wiebe, 1986 \ I n p r e s e n t i n g t h i s t h e s i s i n p a r t i a l f u l f i l m e n t o f t h e r e q u i r e m e n t s f o r an a d v a n c e d d e g r e e a t t h e U n i v e r s i t y o f B r i t i s h C o l u m b i a , I a g r e e t h a t t h e L i b r a r y s h a l l make i t f r e e l y a v a i l a b l e f o r r e f e r e n c e and s t u d y . I f u r t h e r a g r e e t h a t p e r m i s s i o n f o r e x t e n s i v e c o p y i n g o f t h i s t h e s i s f o r s c h o l a r l y p u r p o s e s may be g r a n t e d by t h e h e a d o f my d e p a r t m e n t o r by h i s o r h e r r e p r e s e n t a t i v e s . I t i s u n d e r s t o o d t h a t c o p y i n g o r p u b l i c a t i o n o f t h i s t h e s i s f o r f i n a n c i a l g a i n s h a l l n o t be a l l o w e d w i t h o u t my w r i t t e n p e r m i s s i o n . D e p a r t m e n t o f P> OT/IAJY The U n i v e r s i t y o f B r i t i s h C o l u m b i a 1956 Main Mall V a n c o u v e r , Canada V6T 1Y3 D a t e fa^J 12 tfn DE-6 (3/81) i i ABSTRACT The hyphomycete Pseudozyma p r o l i f i c a Bandoni was grown i n batch and continuous l i q u i d c u l tures t o determine the influence o f growth rate and n u t r i t i o n on conidium development. In batch culture, a period o f elongation and branch formation, followed by fragmentation, was t y p i c a l l y observed. The stage of branch formation was almost completely eliminated when the amino acids phenylalanine, glutamic acid, or asparagine were substituted f o r n i t r a t e . Substituting c i t r i c acid f o…
has type: Thesis/Dissertation
is shown at: 10.14288/1.0096885
language: eng
degree discipline: Botany
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
scholar level: Graduate
type: Text
identifier URI: http://hdl.handle.net/2429/26202