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Alboloshi, Elaf

University of British Columbia

Objectives: The objective of this study was to assess the antimicrobial efficacy of Triton, an all-in-one dual-action endodontic irrigation solution used either independently or sequentially after NaOCl, against multispecies biofilms in dentin canals, with and without the smear layer. Additionally, we aimed to compare its effectiveness with traditionally used solutions (NaOCl / EDTA). Methods: We prepared ten infected dentin specimens with a smear layer and ten specimens without a smear layer. Utilizing a previous protocol, we introduced multispecies bacteria into dentinal tubules. Following a 3-week incubation period, the infected dentin blocks underwent exposure to various irrigating solutions, including sterile water, 2% sodium hypochlorite (NaOCl), 2% NaOCl / 17% EDTA, Triton, and 2% NaOCl / Triton. The proportions of live and dead bacteria were analyzed using confocal laser scanning microscopy and viability staining. Results: The Triton groups (Triton and 2% NaOCl / Triton) exhibited significantly higher bacterial killing compared to 2%NaOCl / EDTA, irrespective of the presence of the smear layer. Furthermore, a more pronounced reduction in bacterial load was observed in groups without a smear layer compared to those with a smear layer. Conclusions: The Triton groups (Triton and 2% NaOCl / Triton) demonstrated the most effective bacterial killing, irrespective of the presence of the smear layer. Triton, the all-in-one, dual-action endodontic irrigation solution, whether used independently or in sequence after 2%NaOCl, displayed superior antimicrobial effects against 3-week-old multispecies biofilms in dentin canals compared to traditionally used 2%NaOCl / EDTA irrigation solutions. The combined application of Triton after 2%NaOCl revealed the highest bacterial killing. This study underscores Triton's heightened and synergistic antimicrobial effect when employed as a final rinse following 2%NaOCl.

Text

2024-05-13

Attribution-NonCommercial-NoDerivatives 4.0 International

http://hdl.handle.net/2429/88288

Craniofacial Science

University of British Columbia

UBCV

http://creativecommons.org/licenses/by-nc-nd/4.0/