Antimicrobial effectiveness of an all-in-one endodontic irrigation solution (Triton) : an in vitro study
department or school: Dentistry, Faculty of
data provider: DSpace
degree campus: UBCV
creator: Alboloshi, Elaf
issued: 2024-05-13T21:39:32Z
issued: 2024
related degree: Master of Science - MSc
degree grantor: University of British Columbia
description: Objectives: The objective of this study was to assess the antimicrobial efficacy of Triton, an all-in-one dual-action endodontic irrigation solution used either independently or sequentially after NaOCl, against multispecies biofilms in dentin canals, with and without the smear layer. Additionally, we aimed to compare its effectiveness with traditionally used solutions (NaOCl / EDTA). Methods: We prepared ten infected dentin specimens with a smear layer and ten specimens without a smear layer. Utilizing a previous protocol, we introduced multispecies bacteria into dentinal tubules. Following a 3-week incubation period, the infected dentin blocks underwent exposure to various irrigating solutions, including sterile water, 2% sodium hypochlorite (NaOCl), 2% NaOCl / 17% EDTA, Triton, and 2% NaOCl / Triton. The proportions of live and dead bacteria were analyzed using confocal laser scanning microscopy and viability staining. Results: The Triton groups (Triton and 2% NaOCl / Triton) exhibited significantly higher bacterial killing compared to 2%NaOCl / EDTA, irrespective of the presence of the smear layer. Furthermore, a more pronounced reduction in bacterial load was observed in groups without a smear layer compared to those with a smear layer. Conclusions: The Triton groups (Triton and 2% NaOCl / Triton) demonstrated the most effective bacterial killing, irrespective of the presence of the smear layer. Triton, the all-in-one, dual-action endodontic irrigation solution, whether used independently or in sequence after 2%NaOCl, displayed superior antimicrobial effects against 3-week-old multispecies biofilms in dentin canals compared to traditionally used 2%NaOCl / EDTA irrigation solutions. The combined application of Triton after 2%NaOCl revealed the highest bacterial killing. This study underscores Triton's heightened and synergistic antimicrobial effect when employed as a final rinse following 2%NaOCl.
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/88288
note: ANTIMICROBIAL EFFECTIVESNESS OF AN ALL-IN-ONE ENDODONTIC IRRIGATION SOLUTION (TRITON): AN IN VITRO STUDY by Elaf Alboloshi BDS, King Abdulaziz University, 2018 A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE in The Faculty of Graduate and Postdoctoral Studies (Craniofacial Science) THE UNIVERSITY OF BRITISH COLUMBIA (Vancouver) May 2024 © Elaf Alboloshi, 2024 ii The following individuals certify that they have read, and recommend to the Faculty of Graduate and Postdoctoral Studies for acceptance, the thesis entitled: Antimicrobial effectiveness of an all-in-one endodontic irrigation solution (Triton): an in vitro study submitted by Elaf Alboloshi in partial fulfilment of the requirements for the degree of Master of Science in Craniofacial Science Examining Committee: Dr. Ya Shen. Professor, Department of Oral Biological & Medical Sciences, UBC Supervisor Dr. Jeffrey Coil. Assistant Professor, Director of the Graduate Endodontic Program, Department of Oral Biological and Medical Sciences, UBC Supervisory Committee Member Dr. Ahmed Hieawy. Clinical Associate Professor, Department of Endodontics, UBC Supervisory Committee Member Dr. Jolanta Aleksejūnienė. Associate Professor, Department of Oral Health Sciences, UBC Additional Examiner iii Abstract Objectives: The objective of this study was to assess the antimicrobial efficacy of Triton, an all-in-one dual-action endodontic irrigation solution used either independently or sequentially after NaOCl, against multispecies biofilms in dentin canals, with and without the smear layer. Additionally, we aimed to compare its effectiveness with traditionally used solutions (NaOCl / EDTA). Methods: We prepared ten infected dentin specimens with a smear layer and ten specimens without a smear layer. Utilizing a previous protocol, we introduced multispecies bacteria into dentinal tubules. Following a 3-week incubation period, the infected dentin blocks underwent exposure to various i…
has type: Thesis/Dissertation
date issued: 2024-11
is shown at: 10.14288/1.0443093
language: eng
degree discipline: Craniofacial Science
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: Attribution-NonCommercial-NoDerivatives 4.0 International
rights URI: http://creativecommons.org/licenses/by-nc-nd/4.0/
scholar level: Graduate
contributor: Shen, Ya
type: Text
identifier URI: http://hdl.handle.net/2429/88288