Open Collections

Li, Shujian

University of British Columbia

The ability to restore tissue architecture and function after an injury is critical to health maintenance. Most studies investigate in this field of research are based on ex vivo histologic samples or genetic analysis of tissue biopsies. Previously in our lab, a real-time multimodality imaging system was developed for continuous monitoring of the exact same microscopic location for skin cellular dynamics. Meanwhile, a new laser therapy technique termed, multiphoton-thermolysis, was developed to achieve precise micro-alteration of skin without affecting the surrounding untargeted tissues. However, multiphoton-thermolysis has not yet been utilized in human skin in vivo. The objective of this thesis was to demonstrate the capability of multiphoton-thermolysis to induce precise skin alteration on human subject and to test the ability of our multimodality microscopy in monitoring skin dynamics in vivo following laser exposure. In this study, five volunteers were recruited, each of the volunteers received two multiphoton-thermolysis sessions on their inner forearm. The following skin response was monitored with the multimodality imaging system which integrates reflectance confocal microscopy (RCM), two-photon excitation fluorescence microscopy (TPF), and second harmonic generation microscopy (SHG) to provide complementary tissue information. Concurrently, an imaging-guided micro-Raman spectroscopy (IMRS) was also incorporated into this system to measure any biochemical changes during the recovery period. Each volunteer was measured at 8 time points, including: before, immediately after, 3 hours, 1 days, 3 days, 1 week, 2 weeks, and 4 weeks after the laser exposure. The results revealed that cellular response, including oncosis, necrosis, and inflammation, and tissue architectural modification including dermal remodeling after laser exposure were all successfully recorded using the multimodality imaging system. This pilot study shows that multiphoton-thermolysis generates tissue alteration and initiates wound healing-like response in human subjects, which may provide a mechanism for treatment of skin conditions including skin cancers.

Text

2023-10-31

Attribution-NonCommercial-NoDerivatives 4.0 International

http://hdl.handle.net/2429/80094

Interdisciplinary Oncology

University of British Columbia

UBCV

http://creativecommons.org/licenses/by-nc-nd/4.0/