Investigation of skin dynamic response after precise multiphoton-thermolysis using non-invasive multimodality microscopy and imaging-guided micro-Raman spectroscopy
department or school: Medicine, Faculty of
data provider: DSpace
degree campus: UBCV
creator: Li, Shujian
issued: 2023-10-31T07:00:00Z
issued: 2021
related degree: Master of Science - MSc
degree grantor: University of British Columbia
description: The ability to restore tissue architecture and function after an injury is critical to health maintenance. Most studies investigate in this field of research are based on ex vivo histologic samples or genetic analysis of tissue biopsies. Previously in our lab, a real-time multimodality imaging system was developed for continuous monitoring of the exact same microscopic location for skin cellular dynamics. Meanwhile, a new laser therapy technique termed, multiphoton-thermolysis, was developed to achieve precise micro-alteration of skin without affecting the surrounding untargeted tissues. However, multiphoton-thermolysis has not yet been utilized in human skin in vivo. The objective of this thesis was to demonstrate the capability of multiphoton-thermolysis to induce precise skin alteration on human subject and to test the ability of our multimodality microscopy in monitoring skin dynamics in vivo following laser exposure. In this study, five volunteers were recruited, each of the volunteers received two multiphoton-thermolysis sessions on their inner forearm. The following skin response was monitored with the multimodality imaging system which integrates reflectance confocal microscopy (RCM), two-photon excitation fluorescence microscopy (TPF), and second harmonic generation microscopy (SHG) to provide complementary tissue information. Concurrently, an imaging-guided micro-Raman spectroscopy (IMRS) was also incorporated into this system to measure any biochemical changes during the recovery period. Each volunteer was measured at 8 time points, including: before, immediately after, 3 hours, 1 days, 3 days, 1 week, 2 weeks, and 4 weeks after the laser exposure. The results revealed that cellular response, including oncosis, necrosis, and inflammation, and tissue architectural modification including dermal remodeling after laser exposure were all successfully recorded using the multimodality imaging system. This pilot study shows that multiphoton-thermolysis generates …
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/80094
note: Investigation of Skin Dynamic Response after Precise Multiphoton-Thermolysis Using Non-invasive Multimodality Microscopy and Imaging-guided Micro-Raman Spectroscopy by Shujian Li H.B.Sc., University of Toronto, 2018 A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE in THE FACULTY OF GRADUATE AND POSTDOCTORAL STUDIES (Interdisciplinary Oncology) THE UNIVERSITY OF BRITISH COLUMBIA (Vancouver) October 2021 © Shujian Li, 2021 ii The following individuals certify that they have read, and recommend to the Faculty of Graduate and Postdoctoral Studies for acceptance, a thesis entitled: Investigation of Skin Dynamic Response after Precise Multiphoton-Thermolysis Using Non-invasive Multimodality Microscopy and Imaging-guided Micro-Raman Spectroscopy submitted by Shujian Li in partial fulfillment of the requirements for the degree of Master of Science in Interdisciplinary Oncology Examining Committee: Dr. Haishan Zeng, Department of Dermatology and Skin Science, UBC Supervisor Dr. Harvey Lui, Department of Dermatology and Skin Science, UBC Supervisory Committee Member Dr. Sunil Kalia, Department of Dermatology and Skin Science, UBC Supervisory Committee Member Dr. Pierre Lane, Interdisciplinary Oncology Program, UBC Additional Examiner iii Abstract The ability to restore tissue architecture and function after an injury is critical to health maintenance. Most studies investigate in this field of research are based on ex vivo histologic samples or genetic analysis of tissue biopsies. Previously in our lab, a real-time multimodality imaging system was developed for continuous monitoring of the exact same microscopic location for skin cellular dynamics. Meanwhile, a new laser therapy technique termed, multiphoton-thermolysis, was developed to achieve precise micro-alteration of skin without affecting the surrounding untargeted tissues. However, multiphoton-thermolysis has not yet been utilized in human skin in vivo. The objective of …
has type: Thesis/Dissertation
date issued: 2021-11
is shown at: 10.14288/1.0402642
language: eng
degree discipline: Interdisciplinary Oncology
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: Attribution-NonCommercial-NoDerivatives 4.0 International
rights URI: http://creativecommons.org/licenses/by-nc-nd/4.0/
scholar level: Graduate
contributor: Zeng, Haishan
type: Text
identifier URI: http://hdl.handle.net/2429/80094