UBC Theses and Dissertations

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UBC Theses and Dissertations

Development of a CARS-based multimodal microscopy system for biological tissue imaging Sheng, Mingyu


Modern advanced optical microscopes are important for disease diagnosis and cancer detection since they provide important biochemical information of the biological specimen with subcellular spatial resolution. In my thesis, I have worked on developing a multimodal microscopy. Imaging by coherent anti-Stokes Raman scattering (CARS) has been widely exploited for studying biological properties of lipid structures that generate strong signals attributed to their C−H stretching vibrations. Multiphoton Microscopy (MPM) imaging is used for exogenous and endogenous fluorophores, ordered non-centrosymmetric molecular assemblies. Reflectance Confocal Microscopy (RCM) can provide overall information on surface tissue structures of the biological specimen. In this thesis, a multimodal optical imaging microscopy system was developed. The system combines three imaging modes (RCM, MPM, and CARS) and is the first of its kind. Previous multimodal systems with MPM and CARS have been developed but none have integrated RCM. This developed system is able to provide 3 distinct microscopic images simultaneously at a frame rate of 1 fps with a digital resolution of 1024×1024 pixels. This multimodal microscopy system uses a single femtosecond pulsed laser, a single microscope objective lens to create three separate beams, aimed at the same region of interest to allow simultaneous display of the three co-registered microscopic contrasts (RCM, MPM and CARS). The resulting contrasts and composite image provide complimentary biochemical and morphological information. This multimodal microscopy system could become a powerful tool for the study of complex biological tissues and for disease diagnosis, especially skin cancer diagnosis.

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