Development of a CARS-based multimodal microscopy system for biological tissue imaging
department or school: Medicine, Faculty of
data provider: DSpace
degree campus: UBCV
creator: Sheng, Mingyu
issued: 2022-10-31T07:00:00Z
issued: 2021
related degree: Master of Science - MSc
degree grantor: University of British Columbia
description: Modern advanced optical microscopes are important for disease diagnosis and cancer detection since they provide important biochemical information of the biological specimen with subcellular spatial resolution. In my thesis, I have worked on developing a multimodal microscopy. Imaging by coherent anti-Stokes Raman scattering (CARS) has been widely exploited for studying biological properties of lipid structures that generate strong signals attributed to their C−H stretching vibrations. Multiphoton Microscopy (MPM) imaging is used for exogenous and endogenous fluorophores, ordered non-centrosymmetric molecular assemblies. Reflectance Confocal Microscopy (RCM) can provide overall information on surface tissue structures of the biological specimen. In this thesis, a multimodal optical imaging microscopy system was developed. The system combines three imaging modes (RCM, MPM, and CARS) and is the first of its kind. Previous multimodal systems with MPM and CARS have been developed but none have integrated RCM. This developed system is able to provide 3 distinct microscopic images simultaneously at a frame rate of 1 fps with a digital resolution of 1024×1024 pixels. This multimodal microscopy system uses a single femtosecond pulsed laser, a single microscope objective lens to create three separate beams, aimed at the same region of interest to allow simultaneous display of the three co-registered microscopic contrasts (RCM, MPM and CARS). The resulting contrasts and composite image provide complimentary biochemical and morphological information. This multimodal microscopy system could become a powerful tool for the study of complex biological tissues and for disease diagnosis, especially skin cancer diagnosis.
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/80053
note: DEVELOPMENT OF A CARS-BASED MULTIMODAL MICROSCOPY SYSTEM FOR BIOLOGICAL TISSUE IMAGING by Mingyu Sheng Ph.D, Fudan University, 2008 A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE in THE FACULTY OF GRADUATE AND POSTDOCTORAL STUDIES (Interdisciplinary Oncology) THE UNIVERSITY OF BRITISH COLUMBIA (Vancouver) October 2021 © Mingyu Sheng, 2021 ii The following individuals certify that they have read, and recommend to the Faculty of Graduate and Postdoctoral Studies for acceptance, a thesis entitled: Development of a CARS-Based Multimodal Microscopy System for Biological Tissue Imaging submitted by Mingyu Sheng in partial fulfillment of the requirements for the degree of Master of Science in Interdisciplinary Oncology Examining Committee: Dr. Haishan Zeng, Department of Dermatology and Skin Science, UBC Supervisor Dr. Sunil Kalia, Department of Dermatology and Skin Science, UBC Supervisory Committee Member Dr. Calum MacAulay, Department of Pathology and Laboratory Medicine, UBC Additional Examiner Additional Supervisory Committee Members: Dr. Harvey Lui, Department of Dermatology and Skin Science, UBC Supervisory Committee Member iii Abstract Modern advanced optical microscopes are important for disease diagnosis and cancer detection since they provide important biochemical information of the biological specimen with subcellular spatial resolution. In my thesis, I have worked on developing a multimodal microscopy. Imaging by coherent anti-Stokes Raman scattering (CARS) has been widely exploited for studying biological properties of lipid structures that generate strong signals attributed to their C−H stretching vibrations. Multiphoton Microscopy (MPM) imaging is used for exogenous and endogenous fluorophores, ordered non-centrosymmetric molecular assemblies. Reflectance Confocal Microscopy (RCM) can provide overall information on surface tissue structures of the biological specimen. In this thesis, a multimodal optical im…
has type: Thesis/Dissertation
date issued: 2021-11
is shown at: 10.14288/1.0402596
language: eng
degree discipline: Interdisciplinary Oncology
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: Attribution-NonCommercial-NoDerivatives 4.0 International
rights URI: http://creativecommons.org/licenses/by-nc-nd/4.0/
scholar level: Graduate
contributor: Zeng, Haishan
type: Text
identifier URI: http://hdl.handle.net/2429/80053