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Identification of miRNAs Involved in Lipid Metabolism and Tuber Development in Cyperus esculentus L. Gao, Yunfei; Wang, Le; Cao, Shanshan; Chen, Liangyu; Li, Xueying; Cong, Weixuan; Yang, Songnan; Zhang, Jian (Professor of biology); Nie, Xiaojun; Zhang, Jun
Abstract
Tiger nut (Cyperus esculentus L.) is recognized for its high oil and oleic acid content in underground tubers. However, the molecular mechanisms governing growth, development, and fatty acid accumulation in these tubers are not well understood. This study employed gas chromatography–mass spectrometry (GC–MS) and small RNA sequencing on tiger nut tubers across five developmental stages. The findings indicate that the critical period for accumulating dry matter and oils, particularly oleic acid, occurs between 35 and 75 days after tuber formation. A total of 183 microRNAs (miRNAs) were identified, comprising 31 known and 152 novel miRNAs. Approximately half of these miRNAs (such as ces-miR156b-3p and ces-miR166a-3p) exhibited differential expression during and around the key periods of metabolite synthesis. The predicted target genes of these miRNAs were significantly enriched in glycerate 3-phosphate metabolism and cell growth processes. Furthermore, 13 miRNA–mRNA interaction modules related to oil accumulation and tuber growth were identified, and these target genes’ expression levels showed significant differences during the tuber developmental stages. These findings advance the understanding of the molecular mechanisms underlying tuber development and oil accumulation in tiger nut.
Item Metadata
| Title |
Identification of miRNAs Involved in Lipid Metabolism and Tuber Development in Cyperus esculentus L.
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| Creator | |
| Publisher |
Multidisciplinary Digital Publishing Institute
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| Date Issued |
2024-11-25
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| Description |
Tiger nut (Cyperus esculentus L.) is recognized for its high oil and oleic acid content in underground tubers. However, the molecular mechanisms governing growth, development, and fatty acid accumulation in these tubers are not well understood. This study employed gas chromatography–mass spectrometry (GC–MS) and small RNA sequencing on tiger nut tubers across five developmental stages. The findings indicate that the critical period for accumulating dry matter and oils, particularly oleic acid, occurs between 35 and 75 days after tuber formation. A total of 183 microRNAs (miRNAs) were identified, comprising 31 known and 152 novel miRNAs. Approximately half of these miRNAs (such as ces-miR156b-3p and ces-miR166a-3p) exhibited differential expression during and around the key periods of metabolite synthesis. The predicted target genes of these miRNAs were significantly enriched in glycerate 3-phosphate metabolism and cell growth processes. Furthermore, 13 miRNA–mRNA interaction modules related to oil accumulation and tuber growth were identified, and these target genes’ expression levels showed significant differences during the tuber developmental stages. These findings advance the understanding of the molecular mechanisms underlying tuber development and oil accumulation in tiger nut.
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| Subject | |
| Genre | |
| Type | |
| Language |
eng
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| Date Available |
2025-01-02
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| Provider |
Vancouver : University of British Columbia Library
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| Rights |
CC BY 4.0
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| DOI |
10.14288/1.0447629
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| URI | |
| Affiliation | |
| Citation |
Plants 13 (23): 3305 (2024)
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| Publisher DOI |
10.3390/plants13233305
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| Peer Review Status |
Reviewed
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| Scholarly Level |
Faculty; Researcher
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| Rights URI | |
| Aggregated Source Repository |
DSpace
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Rights
CC BY 4.0