Identification of miRNAs Involved in Lipid Metabolism and Tuber Development in Cyperus esculentus L.
department or school: Science, Irving K. Barber Faculty of (Okanagan)
department or school: Non UBC
department or school: Biology, Department of (Okanagan)
data provider: DSpace
identifier citation: Plants 13 (23): 3305 (2024)
creator: Gao, Yunfei
creator: Wang, Le
creator: Cao, Shanshan
creator: Chen, Liangyu
creator: Li, Xueying
creator: Cong, Weixuan
creator: Yang, Songnan
creator: Zhang, Jian (Professor of biology)
creator: Nie, Xiaojun
creator: Zhang, Jun
issued: 2025-01-02T18:37:25Z
issued: 2024-11-25
description: Tiger nut (Cyperus esculentus L.) is recognized for its high oil and oleic acid content in underground tubers. However, the molecular mechanisms governing growth, development, and fatty acid accumulation in these tubers are not well understood. This study employed gas chromatography–mass spectrometry (GC–MS) and small RNA sequencing on tiger nut tubers across five developmental stages. The findings indicate that the critical period for accumulating dry matter and oils, particularly oleic acid, occurs between 35 and 75 days after tuber formation. A total of 183 microRNAs (miRNAs) were identified, comprising 31 known and 152 novel miRNAs. Approximately half of these miRNAs (such as ces-miR156b-3p and ces-miR166a-3p) exhibited differential expression during and around the key periods of metabolite synthesis. The predicted target genes of these miRNAs were significantly enriched in glycerate 3-phosphate metabolism and cell growth processes. Furthermore, 13 miRNA–mRNA interaction modules related to oil accumulation and tuber growth were identified, and these target genes’ expression levels showed significant differences during the tuber developmental stages. These findings advance the understanding of the molecular mechanisms underlying tuber development and oil accumulation in tiger nut.
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/90004
note: Citation: Gao, Y.; Wang, L.; Cao, S.;Chen, L.; Li, X.; Cong, W.; Yang, S.;Zhang, J.; Nie, X.; Zhang, J.Identification of miRNAs Involved inLipid Metabolism and TuberDevelopment in Cyperus esculentus L.Plants 2024, 13, 3305. https://doi.org/10.3390/plants13233305Academic Editor: AlexandraS. DubrovinaReceived: 7 October 2024Revised: 13 November 2024Accepted: 20 November 2024Published: 25 November 2024Copyright: © 2024 by the authors.Licensee MDPI, Basel, Switzerland.This article is an open access articledistributed under the terms andconditions of the Creative CommonsAttribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).ArticleIdentification of miRNAs Involved in Lipid Metabolism andTuber Development in Cyperus esculentus L.Yunfei Gao 1,†, Le Wang 2,† , Shanshan Cao 1, Liangyu Chen 1 , Xueying Li 1, Weixuan Cong 1 , Songnan Yang 1 ,Jian Zhang 1,3, Xiaojun Nie 4,* and Jun Zhang 1,5,*1 College of Agronomy, Jilin Agricultural University, Changchun 130118, China;20210055@mails.jlau.edu.cn (Y.G.); zhangjian@jlau.edu.cn (J.Z.)2 College of Plant Science, Jilin University, Changchun 130062, China; lewang27@jlu.edu.cn3 Department of Biology, University of British Columbia, Okanagan, Kelowna, BC V1V 1V7, Canada4 State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, College of Agronomy,Northwest A&F University, Xianyang 712100, China5 National Crop Variety Approval and Characteristic Identification Station, Jilin Agricultural University,Changchun 130118, China* Correspondence: small@nwsuaf.edu.cn (X.N.); zhangjun@jlau.edu.cn (J.Z.)† These authors contributed equally to this work.Abstract: Tiger nut (Cyperus esculentus L.) is recognized for its high oil and oleic acid content inunderground tubers. However, the molecular mechanisms governing growth, development, and fattyacid accumulation in these tubers are not well understood. This study employed gas chromatography–mass spectrometry (GC–MS) and small RNA sequencing on tiger nut tube…
has type: Article
is shown at: 10.14288/1.0447629
language: eng
peer review status: Reviewed
provider: Vancouver : University of British Columbia Library
publisher: Multidisciplinary Digital Publishing Institute
publisher DOI: 10.3390/plants13233305
rights: CC BY 4.0
rights URI: https://creativecommons.org/licenses/by/4.0/
scholar level: Faculty
scholar level: Researcher
subject: Cyperus esculentus
subject: miRNAs
subject: GC-MS
subject: oil synthesis
subject: tuber development
type: Text
identifier URI: http://hdl.handle.net/2429/90004