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Singh, Sharandeep

University of British Columbia

Fusarium head blight (FHB) is an important fungal disease affecting the yield and quality of wheat. Deploying genetic resistance in wheat is an essential component of an integrated strategy for reducing the adverse effects of the disease. Most previous studies have mapped FHB resistance from Chinese or Brazilian germplasm. In preliminary research, 12 Watkins wheat landraces with MR/R resistance to FHB severity and deoxynivalenol (DON) toxin accumulation were identified. In the present study, we are utilizing one of the 12 Watkins landraces identified, namely, Wat.1190580 (origin: Iran) to identify gene(s)/QTL for FHB and DON resistance. Wat.1190580 has a resistant reaction to FHB and DON accumulation and is used as a male/resistance donor parent to develop recombinant inbred lines (RIL) population: Paragon X Wat.1190580 (F8, n=75). Paragon is a European spring wheat cultivar susceptible to FHB. The mapping population was screened at the UBC Greenhouse in (2021) and in field nurseries in Morden (2021, 2022, 2023), and Carman (2021, 2022) for FHB infection: incidence (INC), severity (SEV), Fusarium damaged kernels (FDKs), and DON and phenological traits: plant height (PHT), days to anthesis (DTA). At first, Paragon X Wat.1190580 was genotyped using a Wheat 90K iSelect SNP array. Our results from 90K genotyping resulted in genetic maps with large intervals between skeleton markers (absence of markers in most regions across the entire genome) indicating allelic diversity in landraces not represented by the Wheat 90K iSelect SNP array. As 90K is identified as unsuitable for genotyping of the RILs derived from Watkin landraces, we genotyped the population using a skim-sequencing approach at 0.1x coverage and generated genome assembly of Wat.1190580 (20x coverage). QTL regions associated with INC (2B, 5A), SEV (2B, 3B, 5B), DON (5A), FDKs (5A) resistance, and PHT (3A, 5A), and DTA (5A) were successfully mapped using the phenotypic data and the skim-sequencing data.

Text

2025-04-30

Attribution-NonCommercial-NoDerivatives 4.0 International

http://hdl.handle.net/2429/88063

Plant Science

University of British Columbia

UBCV

http://creativecommons.org/licenses/by-nc-nd/4.0/