Genetic mapping of resistance to Fusarium head blight and DON accumulation in Watkins landrace Wat.1190580
department or school: Land and Food Systems, Faculty of
data provider: DSpace
degree campus: UBCV
creator: Singh, Sharandeep
issued: 2025-04-30T07:00:00Z
issued: 2024
related degree: Master of Science - MSc
degree grantor: University of British Columbia
description: Fusarium head blight (FHB) is an important fungal disease affecting the yield and quality of wheat. Deploying genetic resistance in wheat is an essential component of an integrated strategy for reducing the adverse effects of the disease. Most previous studies have mapped FHB resistance from Chinese or Brazilian germplasm. In preliminary research, 12 Watkins wheat landraces with MR/R resistance to FHB severity and deoxynivalenol (DON) toxin accumulation were identified. In the present study, we are utilizing one of the 12 Watkins landraces identified, namely, Wat.1190580 (origin: Iran) to identify gene(s)/QTL for FHB and DON resistance. Wat.1190580 has a resistant reaction to FHB and DON accumulation and is used as a male/resistance donor parent to develop recombinant inbred lines (RIL) population: Paragon X Wat.1190580 (F8, n=75). Paragon is a European spring wheat cultivar susceptible to FHB. The mapping population was screened at the UBC Greenhouse in (2021) and in field nurseries in Morden (2021, 2022, 2023), and Carman (2021, 2022) for FHB infection: incidence (INC), severity (SEV), Fusarium damaged kernels (FDKs), and DON and phenological traits: plant height (PHT), days to anthesis (DTA). At first, Paragon X Wat.1190580 was genotyped using a Wheat 90K iSelect SNP array. Our results from 90K genotyping resulted in genetic maps with large intervals between skeleton markers (absence of markers in most regions across the entire genome) indicating allelic diversity in landraces not represented by the Wheat 90K iSelect SNP array. As 90K is identified as unsuitable for genotyping of the RILs derived from Watkin landraces, we genotyped the population using a skim-sequencing approach at 0.1x coverage and generated genome assembly of Wat.1190580 (20x coverage). QTL regions associated with INC (2B, 5A), SEV (2B, 3B, 5B), DON (5A), FDKs (5A) resistance, and PHT (3A, 5A), and DTA (5A) were successfully mapped using the phenotypic data and the skim-sequencing data.
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/88063
note: Genetic mapping of resistance to Fusarium head blight and DON accumulation in Watkins landrace Wat.1190580 by Sharandeep Singh B.Sc., Punjab Agricultural University, 2020 A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE in THE FACULTY OF GRADUATE AND POSTDOCTORAL STUDIES (Plant Science) THE UNIVERSITY OF BRITISH COLUMBIA (Vancouver) April, 2024 © Sharandeep Singh, 2024 ii The following individuals certify that they have read, and recommend to the Faculty of Graduate and Postdoctoral Studies for acceptance, the thesis entitled: Genetic mapping of resistance to Fusarium head blight and DON accumulation in Watkins landrace Wat.1190580 submitted by Sharandeep Singh in partial fulfilment of the requirements for the degree of Master of Science in Plant Science Examining Committee: Dr. Andrew Riseman, Associate Professor, Applied Biology, UBC Supervisor Dr. Gurcharn S. Brar, Assistant Professor, Wheat Breeding and Genetics, University of Alberta Supervisory Committee Member Dr. Harpinder Randhawa, Research Scientist, Spring Wheat Breeding, Agriculture and Agri-Food Canada Additional Examiner Additional Supervisory Committee Members: Dr. Rishi R. Burlakoti, Research Scientist, Plant Pathology, Agriculture and Agri-Food Canada Supervisory Committee Member iii Abstract Fusarium head blight (FHB) is an important fungal disease affecting the yield and quality of wheat. Deploying genetic resistance in wheat is an essential component of an integrated strategy for reducing the adverse effects of the disease. Most previous studies have mapped FHB resistance from Chinese or Brazilian germplasm. In preliminary research, 12 Watkins wheat landraces with MR/R resistance to FHB severity and deoxynivalenol (DON) toxin accumulation were identified. In the present study, we are utilizing one of the 12 Watkins landraces identified, namely, Wat.1190580 (origin: Iran) to identify gene(s)/QTL for FHB and DON resistance. Wat.1190580 has a resistan…
has type: Thesis/Dissertation
date issued: 2024-05
is shown at: 10.14288/1.0442031
language: eng
degree discipline: Plant Science
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: Attribution-NonCommercial-NoDerivatives 4.0 International
rights URI: http://creativecommons.org/licenses/by-nc-nd/4.0/
scholar level: Graduate
contributor: Riseman, Andrew
type: Text
identifier URI: http://hdl.handle.net/2429/88063