UBC Undergraduate Research

Artificial inoculation of Phytophthora ramorum on Pseudotsuga menziesii and Larix kamperfi bolts to assess lineage aggression, survival and detection methods Sheppard, Julie


This experiment tested the survival, and aggression of the four different lineages of P. ramorum (NA1, NA2, EU1, EU2) on Douglas fir bolts. The average lesions size produced at 4 weeks was 7.47cm, at 8 weeks was 13.125cm and at 12 weeks was 6.18cm. There was no significant difference in aggression between the four lineages, but the EU2 lineage produced the largest lesions after 4 weeks with the most variability (8.375±1.99). Re-isolation of the pathogen was poor, with only 16% success after 4 weeks, 4% after 8 weeks and 0% after 12 weeks. Molecular techniques were better able to detect the pathogen, showing presence of the pathogen 9 weeks after exposure to air. This however does not indicate viability and further analysis needs to be done to confirm survival of the pathogen over time. Overall this experiment confirms the susceptibility of Douglas fir to all four lineages of P. ramorum, and indicates that there is potential for survival on logs.

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