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Expression of C. fimi genes from transposon Tn10 tet promoters Din, Neena
Abstract
In this study, the promoterless genes cenA and cex (both obtained from E. coli expressing recombinant DNA of C. fimh were placed under the control of the divergent tet promoters from Tn10 and cloned into a broad host range plasmid, pJRD215. This system allowed us to investigate the expression and interaction of the gene products of these two genes (CenA and Cex) in four different Gram-negative host organisms: E. coli C600, an E. coli K12 derivative which is able to survive on cellobiose, R. capsulatus B10, and K. pneumoniae M5a1. The objective was to eventually find a Gram-negative host organism which would secrete the CenA and Cex proteins so that we could investigate whether it was now possible for that host organism to survive on cellulose. All four host organisms used in this study expressed the cenA and the cex genes with different efficiencies, however, none of the host organisms secreted CenA and Cex to the culture medium.
Item Metadata
Title |
Expression of C. fimi genes from transposon Tn10 tet promoters
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Creator | |
Publisher |
University of British Columbia
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Date Issued |
1989
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Description |
In this study, the promoterless genes cenA and cex (both obtained from E. coli expressing recombinant DNA of C. fimh were placed under the control of the divergent tet promoters from Tn10 and cloned into a broad host range plasmid, pJRD215. This system allowed us to investigate the expression and interaction of the gene products of these two genes (CenA and Cex) in four different Gram-negative host organisms: E. coli C600, an E. coli K12 derivative which is able to survive on cellobiose, R. capsulatus B10, and K. pneumoniae M5a1. The objective was to eventually find a Gram-negative host organism which would secrete the CenA and Cex proteins so that we could investigate whether it was now possible for that host organism to survive on cellulose.
All four host organisms used in this study expressed the cenA and the cex genes with different efficiencies, however, none of the host organisms secreted CenA and Cex to the culture medium.
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Type | |
Language |
eng
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Date Available |
2010-08-16
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Provider |
Vancouver : University of British Columbia Library
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Rights |
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
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DOI |
10.14288/1.0302136
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URI | |
Degree | |
Program | |
Affiliation | |
Degree Grantor |
University of British Columbia
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Campus | |
Scholarly Level |
Graduate
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Aggregated Source Repository |
DSpace
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Rights
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.