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A chromatograhic investigation of the alcohol-soluble nitrogenous constituents of Verticillium albo-atrum R. and B. isolates Pepin, Herbert Spencer

Abstract

Verticillium albo-atrum R. and B. isolates were separated into four strain groups based on morphological type. All isolates were tested for pathogenicity toward tomato, and an attempt was made to relate pathogenicity to morphological type. Since structural and functional differences in plants and animals are believed to be invariably associated with chemical differences, a survey of the nitrogenous constituents of the isolates was made in an attempt to relate any differences to morphological or pathologic strains. The alcohol-soluble extract from the mycelium of each isolate was investigated chromatographically for amino acids, amines, amides and peptides. The amino acids found were the same for all isolates. These were aspartic acid, glutamic acid, lysine, histidine, isoleucine, serine, valine, proline, methionine, ∝ alanine, tyrosine, threonine, glycine and one unidentified neutral compound, A. No amines, amides or peptides were detected. The amino acids, amines, amides and peptides of the ∝, β and Ȣ strains of Colletotricum lindemuthianum (Sacc. and Magn. ) Bri. and Gav. were isolated and identified using the techniques of paper chromatography for the purpose of comparison with Verticillium albo-atrum. The three Colletotricum strains contained the same amino acids as the Verticillium isolates with the exception of unknown A which was replaced by another unknown, B, common to all three Colletotricum strains indicating a definite species difference. Strain differences apparently do not occur in these two organisms in the groups of compounds studied. The effect of aeration, age of mycelium, pH of medium and carbon source on the qualitative amino acid content of isolate V3 were studied. These environmental conditions had no qualitative effect on the amino acids of the isolate. The free nucleotides of the acid-soluble extract of six representative isolates were studied using the technique of anion-exchange chromatography. Nucleotides isolated from all isolates and tentatively identified were guanosine monophosphate, guanosine diphosphate, guanosine triphosphate, cyt-idine diphosphate, uridine monophosphate, uridine diphosphate, uridine triphosphate and adenosine diphosphate. No other nucleotides were isolated. The above results indicated that if chemical dif-ferences do exist between strains, as postulated, they do not occur, In these two organisms, among the nitrogenous compounds studied, but must occur In some other class of compounds not studied in this investigation.

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