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The purification and properties of ribonucleases Roy, Kenneth Leo Joseph
Abstract
The purification of ribonucleases T₁ and T₂ from Ta-kadiastase has been carried out to provide enzymes of high purity and known specificity for structural studies on s-RNA. These purifications involved acid treatment, acetone fractionation, gel filtration on Sephadex and ion-exchange chromatography on substituted celluloses. Satisfactorily pure RNase T₁ has been obtained and its specificity has been confirmed. Further purification of RNase T₂ might be desireable but some studies using chemically synthesized substrates have been carried out on the most highly purified fraction yet obtained. Partial purification of an RNase present in a different diastase preparation involved heat, acid, acetone fractionation and anion exchange chromatography. Information is not yet available on its specificity because of the small quantities isolated.
Item Metadata
Title |
The purification and properties of ribonucleases
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Creator | |
Publisher |
University of British Columbia
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Date Issued |
1964
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Description |
The purification of ribonucleases T₁ and T₂ from Ta-kadiastase has been carried out to provide enzymes of high purity and known specificity for structural studies on s-RNA. These purifications involved acid treatment, acetone fractionation, gel filtration on Sephadex and ion-exchange chromatography on substituted celluloses. Satisfactorily pure RNase T₁ has been obtained and its specificity has been confirmed. Further purification of RNase T₂ might be desireable but some studies using chemically synthesized substrates have been carried out on the most highly purified fraction yet obtained.
Partial purification of an RNase present in a different diastase preparation involved heat, acid, acetone fractionation and anion exchange chromatography. Information is not yet available on its specificity because of the small quantities isolated.
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Genre | |
Type | |
Language |
eng
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Date Available |
2011-12-16
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Provider |
Vancouver : University of British Columbia Library
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Rights |
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
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DOI |
10.14288/1.0106003
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URI | |
Degree | |
Program | |
Affiliation | |
Degree Grantor |
University of British Columbia
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Campus | |
Scholarly Level |
Graduate
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Aggregated Source Repository |
DSpace
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Rights
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.