UBC Theses and Dissertations

UBC Theses Logo

UBC Theses and Dissertations

The purification and properties of ribonucleases Roy, Kenneth Leo Joseph

Abstract

The purification of ribonucleases T₁ and T₂ from Ta-kadiastase has been carried out to provide enzymes of high purity and known specificity for structural studies on s-RNA. These purifications involved acid treatment, acetone fractionation, gel filtration on Sephadex and ion-exchange chromatography on substituted celluloses. Satisfactorily pure RNase T₁ has been obtained and its specificity has been confirmed. Further purification of RNase T₂ might be desireable but some studies using chemically synthesized substrates have been carried out on the most highly purified fraction yet obtained. Partial purification of an RNase present in a different diastase preparation involved heat, acid, acetone fractionation and anion exchange chromatography. Information is not yet available on its specificity because of the small quantities isolated.

Item Media

Item Citations and Data

Rights

For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.