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Studies on the active region of botulinus toxins : involvement and sequence of a peptide in the region of the single cysteine residue in botulinus toxins types A, B and E Van Alstyne, Diane
Abstract
After establishing that, like types B and E toxins, type A botulinus toxin contains only one residue of cysteine per molecule, chemical modification studies were carried out showing that sulfhydryl-specific reagents like p-chloromercuribenzoate (PCMB) and N-(4-dimethyl-amino-3,5-dinitrophenyl) maleimide (DDPM) cause a marked decrease in toxicity. Types A, B and E toxins were reacted with DDPM and a labelled peptide was obtained from the tryptic digest of each of the toxins. The quantitative amino acid analyses of these three peptides were remarkably similar. Sequence analyses showed that a sequence ala-glu-ser-cys-ser-asp-ser is common to all three DDPS-peptides. Type B botulinus toxin does not enzymatically alter acetylcholine in order to cause flaccid paralysis. It is postulated that the three thiol-containing peptides isolated contain all or part of a postulated active site common to each of types A, B and E botulinus toxins.
Item Metadata
Title |
Studies on the active region of botulinus toxins : involvement and sequence of a peptide in the region of the single cysteine residue in botulinus toxins types A, B and E
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Creator | |
Publisher |
University of British Columbia
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Date Issued |
1966
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Description |
After establishing that, like types B and E toxins, type A botulinus toxin contains only one residue of cysteine per molecule, chemical modification studies were carried out showing that sulfhydryl-specific reagents like p-chloromercuribenzoate (PCMB) and N-(4-dimethyl-amino-3,5-dinitrophenyl) maleimide (DDPM) cause a marked decrease in toxicity.
Types A, B and E toxins were reacted with DDPM and a labelled peptide was obtained from the tryptic digest of each of the toxins. The quantitative amino acid analyses of these three peptides were remarkably similar. Sequence analyses showed that a sequence
ala-glu-ser-cys-ser-asp-ser is common to all three DDPS-peptides.
Type B botulinus toxin does not enzymatically alter acetylcholine
in order to cause flaccid paralysis.
It is postulated that the three thiol-containing peptides isolated contain all or part of a postulated active site common to each of types A, B and E botulinus toxins.
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Genre | |
Type | |
Language |
eng
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Date Available |
2011-09-19
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Provider |
Vancouver : University of British Columbia Library
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Rights |
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
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DOI |
10.14288/1.0104794
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URI | |
Degree | |
Program | |
Affiliation | |
Degree Grantor |
University of British Columbia
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Campus | |
Scholarly Level |
Graduate
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Aggregated Source Repository |
DSpace
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Item Media
Item Citations and Data
Rights
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.