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Analysis of the spleen colony-forming ability and lympho-myeloid repopulating ability of adherent marrow

University of British Columbia

In an attempt to characterize different populations of primitive hemopoietic stem cells, the spleen colony forming and in vivo lympho-myeloid repopulating ability of bone marrow cells which adhere to plastic was assessed. The property of adherence was chosen for evaluation because it had previously been reported that adherent stem cells have greater self-renewal capacity than non-adherent stem cells (1). When adherent marrow was assessed for spleen colony-forming ability, it contained 2 to 3 fold fewer day 10 CFU-S than fresh marrow but a relatively increased myeloid repopulating ability. These results are consistent with evidence that high self-renewal stem cells form spleen colonies that do not become macroscopically visible until 12 to 14 days post-transplantation (2). The results also suggested that adherence did not enrich for stem cells with long-term T lymphoid repopulating ability, although this may have been generally compromised by minor histocompatibility differences between the donor and host strains used. These studies thus provide further evidence of heterogeneity amongst primitive pluripotent marrow progenitor cells. Adherence separation appears to enrich for a sub-population that does not form spleen colonies within 10 days, but has superior myeloid repopulating potential.

Text

2010-05-15

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http://hdl.handle.net/2429/24700

Medical Genetics

University of British Columbia

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