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UBC Theses and Dissertations

Effect of herbicides on proteins in spring wheat Desai, Prabhat Dinkar


Three varieties of spring wheat, Selkirk, Manitou and Pembina, were grown in the field and greenhouse. 2,4-D, bromoxynil and picloram were applied at the 6" stage (4 leaf). The experiments were carried out in a randomized block design. 2,4-D and picloram showed a low concentration stimulation and high concentration inhibition on yield with Selkirk. Manitou and Pembina did not show definite trends with these two herbicides. Bromoxynil decreased the yield in all three varieties and the decrease was enhanced at higher concentrations. Bromoxynil was the only herbicide that increased the percent protein in all three varieties. An inverse relation between the percent protein and yield was seen in Selkirk, Manitou and Pembina treated with bromoxynil. 2,4-D and picloram also produced this relationship in Selkirk but not in Manitou or Pembina. Bromoxynil significantly reduced the yield of protein per plot, whereas 2,4-D and picloram did not. There was no quantitative relation between the protein extracted by 0.1 M Tris-glycine buffer (pH 8.3) or 0.05 N acetic acid (pH 3.5) and the percent protein in wheat. Selkirk was least affected by any of the herbicides, i.e. to the changes in the protein (albumin, globulin and gluten) as seen from the densitometric traces of gels. Manitou and Pembina showed changes as a result of the bromoxynil treatment.

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