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Radioimmunoassay of salmon calcitonin Bass, Sydney
Abstract
The development of a radioimmunoassay for salmon calcitonin is described. Synthetic salmon calcitonin was iodinated with I(125) and used as tracer. The coated charcoal method, suitably modified, was employed to separate bound and free salmon calcitonin. Antisera were raised by intradermal and intramuscular injection of calcitonin conjugated to keyhole limpet hemocyanin with carbodiimide. The sensitivity of the assay is 50 - 60 pg/ml of incubate. The disappearance of synthetic salmon calcitonin in rainbow trout was determined, illustrating two components, an initial rapid decline followed by a prolonged drop in concentration.
Item Metadata
Title |
Radioimmunoassay of salmon calcitonin
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Creator | |
Publisher |
University of British Columbia
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Date Issued |
1970
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Description |
The development of a radioimmunoassay for salmon calcitonin is described. Synthetic salmon calcitonin was iodinated with I(125) and used as tracer. The coated charcoal method, suitably modified, was employed to separate bound and free salmon calcitonin. Antisera were raised by intradermal and intramuscular injection of calcitonin conjugated to keyhole limpet hemocyanin with carbodiimide. The sensitivity of the assay is 50 - 60 pg/ml of incubate. The disappearance of synthetic salmon calcitonin in rainbow trout was determined, illustrating two components, an initial rapid decline followed by a prolonged drop in concentration.
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Genre | |
Type | |
Language |
eng
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Date Available |
2011-05-12
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Provider |
Vancouver : University of British Columbia Library
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Rights |
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
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DOI |
10.14288/1.0102060
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URI | |
Degree | |
Program | |
Affiliation | |
Degree Grantor |
University of British Columbia
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Campus | |
Scholarly Level |
Graduate
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Aggregated Source Repository |
DSpace
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Item Media
Item Citations and Data
Rights
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.