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Radioimmunoassay of salmon calcitonin Bass, Sydney

Abstract

The development of a radioimmunoassay for salmon calcitonin is described. Synthetic salmon calcitonin was iodinated with I(125) and used as tracer. The coated charcoal method, suitably modified, was employed to separate bound and free salmon calcitonin. Antisera were raised by intradermal and intramuscular injection of calcitonin conjugated to keyhole limpet hemocyanin with carbodiimide. The sensitivity of the assay is 50 - 60 pg/ml of incubate. The disappearance of synthetic salmon calcitonin in rainbow trout was determined, illustrating two components, an initial rapid decline followed by a prolonged drop in concentration.

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