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Differentiation of the chick oviduct in vitro

University of British Columbia

Immature chick oviducts differentiate in vivo in response to daily injections of DES. This is indicated by increases in general oviduct protein nitrogen: DNA ratios and the production of immunologically precipitable ovalbumin. Three types of ovalbumin are produced in response to estrogen. These results indicate that ovalbumin free of phosphorus appears first in the oviduct tissue in response to DES. The appearance of phosphorus containing ovalbumin follows. Attempts were made to induce the in vitro differentiation of oviduct tissue under a variety of culture conditions. A standard culture media was supplemented with DES and serum (fetal calf, cockerel, laying hen, or chick serum) as well as serum from DES injected chicks. No changes were observed in the tissue histology nor in the production of the specific protein, ovalbumin, under the above conditions. Closely associated tissues such as kidneys, ovaries, mesentary or ureter tissue cultured with the oviduct tissue failed to elicite any detectible morphological and chemical changes in the latter. The addition of insulin or homogenized pituitaries from DES injected or non-injected chicks was ineffective as an inducer of differentiation in this system. Substituting 17 6-estradiol for DES as an inducer did not alter the results. Thus, with the exception of estrogen any factor(s) necessary for the in vivo differentiation of immature oviduct tissue remains unknown at this time.

Text

2011-04-07

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http://hdl.handle.net/2429/33372

Animal Science

University of British Columbia

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