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Calcium dependent astrocyte-neuron communication Stringer, Charles Edward Alexander
Abstract
The discovery of direct communication between astrocytes and neurons has changed the perception of astrocytes from passive support cells to active partners in information processing. Astrocytes express myriad neurotransmitter receptors and have been shown to release neurotransmitters, allowing these cells to respond and signal to adjacent neurons, respectively. Astrocytes can rapidly respond to neurotransmitters with a rise in [Ca²⁺]i and astrocyte neurotransmitter release has been shown to be calcium dependent. The purpose of this research was to investigate unknown aspects of astrocyteneuron communication with in situ calcium imaging to improve our understanding of how these cells interact functionally. The first objective of this research was to determine whether extracellular dopamine elicits astrocyte calcium transients in the prefrontal cortex (PFC). Astrocytes from this area express dopamine receptors and dopamine is an important neurotransmitter in the PFC. We found that astrocytes in PFC brain slices reliably respond to a high concentration of dopamine ([50μM]) with [Ca²⁺]i transients, however these responses were due to the activation of adrenoreceptors, not dopamine receptors. The inability of a lower concentration of dopamine ([10μM]) to elicit astrocyte [Ca²⁺]i transients questions the whether these cells can rapidly respond to PFC dopamine at physiological levels. The second objective of this research was to investigate the extent that calcium dependent glutamate release from astrocytes is able to influence neural activity. The best studied mechanism of astrocyte gliotransmitter release is the calcium dependent release of glutamate which has been demonstrated in single astrocytes in situ. We used the vasoactive peptide endothelin to preferentially elicit widespread astrocyte [Ca²⁺]i transients astrocytes from hippocampal brain slices to determine whether the widespread calcium rise in astrocytes was associated with a change in glutamate sensitive synaptic transmission. Despite eliciting nearly ubiquitous astrocyte calcium responses, we observed no change in glutamate sensitive synaptic transmission as measured by extracellular field recordings. These results question the ability of astrocytes to acutely influence synaptic transmission of a brain region. Our findings do not support an acute role of calcium dependent communication between astrocytes and neurons in rapid information processing in the systems we investigated.
Item Metadata
Title |
Calcium dependent astrocyte-neuron communication
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Creator | |
Publisher |
University of British Columbia
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Date Issued |
2007
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Description |
The discovery of direct communication between astrocytes and neurons has
changed the perception of astrocytes from passive support cells to active partners in
information processing. Astrocytes express myriad neurotransmitter receptors and have
been shown to release neurotransmitters, allowing these cells to respond and signal to
adjacent neurons, respectively. Astrocytes can rapidly respond to neurotransmitters with a
rise in [Ca²⁺]i and astrocyte neurotransmitter release has been shown to be calcium
dependent. The purpose of this research was to investigate unknown aspects of astrocyteneuron
communication with in situ calcium imaging to improve our understanding of
how these cells interact functionally.
The first objective of this research was to determine whether extracellular
dopamine elicits astrocyte calcium transients in the prefrontal cortex (PFC). Astrocytes
from this area express dopamine receptors and dopamine is an important neurotransmitter
in the PFC. We found that astrocytes in PFC brain slices reliably respond to a high
concentration of dopamine ([50μM]) with [Ca²⁺]i transients, however these responses
were due to the activation of adrenoreceptors, not dopamine receptors. The inability of a
lower concentration of dopamine ([10μM]) to elicit astrocyte [Ca²⁺]i transients questions
the whether these cells can rapidly respond to PFC dopamine at physiological levels.
The second objective of this research was to investigate the extent that calcium
dependent glutamate release from astrocytes is able to influence neural activity. The best
studied mechanism of astrocyte gliotransmitter release is the calcium dependent release
of glutamate which has been demonstrated in single astrocytes in situ. We used the vasoactive peptide endothelin to preferentially elicit widespread astrocyte [Ca²⁺]i
transients astrocytes from hippocampal brain slices to determine whether the widespread
calcium rise in astrocytes was associated with a change in glutamate sensitive synaptic
transmission. Despite eliciting nearly ubiquitous astrocyte calcium responses, we
observed no change in glutamate sensitive synaptic transmission as measured by
extracellular field recordings. These results question the ability of astrocytes to acutely
influence synaptic transmission of a brain region.
Our findings do not support an acute role of calcium dependent communication
between astrocytes and neurons in rapid information processing in the systems we
investigated.
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Genre | |
Type | |
Language |
eng
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Date Available |
2011-03-22
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Provider |
Vancouver : University of British Columbia Library
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Rights |
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
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DOI |
10.14288/1.0101300
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URI | |
Degree | |
Program | |
Affiliation | |
Degree Grantor |
University of British Columbia
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Campus | |
Scholarly Level |
Graduate
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Aggregated Source Repository |
DSpace
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Rights
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.