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The effect of nutrients on the rate of protein degradation in isolated small intestinal enterocytes from Sprague-Dawley rats Taghadomy Saberi, Mohsen

Abstract

Nutrients affect small intestinal protein mass and metabolism. Studies on the effect of nutrients on small intestinal protein degradation are limited due to a lack of a proper method. The objectives of this study were to establish a method to directly estimate protein degradation in isolated enterocytes from rats, and to test the effect of energy substrates and amino acids on protein degradation. Male Sprague-Dawley rats (150-200 g, n ≥8 per treatment) were used. Cell viability, tyrosine release as an indicator of protein degradation, and the effect of osmolarity were measured to establish the method. The effects of energy substrates (50 mM glucose, 20 mM (β-hydroxybutyrate, 4.7 mM butyrate, and 30 mM glutamine) and amino acid solutions (30 mM amino acids mix, 30 mM glutamate, 1% ammonium, and 30 mM amino acids mix plus 50 mM glucose) on protein degradation were measured. Average viability at time 30 min was 85.8% (range 81-94%). Tyrosine release was linear over the course of experiments, indicating constant protein degradation (R²= 0.9943) (p

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