UBC Theses and Dissertations
Development of pyruvate dehydrogenase in white fat, brown fat and liver of the rat Bailey, Kathryn Anne
The total activity and the fraction of the enzyme in the active form of pyruvate dehydrogenase was assayed in white fat, brown fat and liver throughout the development of the rat. In white adipose tissue, the total activity of pyruvate dehydrogenase and the fraction of the enzyme in the active form did not change significantly during development. In brown adipose tissue, the total activity increased until the late suckling period. After weaning, a decrease was noted. The fraction of the enzyme in the active form did not increase until after ten days of age, reached its highest level in the late suckling period and remained at this level after weaning. Pyruvate dehydrogenase in liver decreased in both total activity and percentage activity in the early neonatal period. Both parameters increased after this period, reaching their highest levels in the late suckling period. In both fetal liver and fetal brown fat, the total activity of pyruvate dehydrogenase was increased by in vitro incubation with insulin.
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