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Behavioral analysis of memory in Caenorhabditis elegans Rose, Jacqueline Kyoko
Abstract
Memory has been the focus of much research for more than 100 years. The earliest documented memory experiments were published by Hermann Ebbinghaus in 1885. In the current studies I investigated parameters affecting memory for habituation in the nematode Caenorhabditis elegans and determined several cellular mechanisms that underlie the behavioral expression of memory. I found that long-term memory for habituation requires a distributed training protocol. I confirmed the generally accepted view that mechanisms of memory consolidation occur after each training trial by showing that protein synthesis inhibition delivered after every training trial effectively blocked long-term memory. Glutamate has been postulated to play an important cellular role in memory consolidation; the findings of these studies confirm that long-term memory for habituation in C. elegans does not occur i f glutamate transmission is compromised. I found that GLR-1 receptor activation is necessary for formation of long-term memory. Further, using a GFP transgenic strain to measure GLR-1 expression, I observed a significant decrease in GLR-1 24 hours after distributed training thus providing a putative mechanism for memory. I examined the permanence of this glutamate dependent long-term memory for habituation by inhibiting protein synthesis during memory reconsolidation and found that this effectively erased the earlier consolidated memory. Finally, I examined memory at an earlier time point (12 hours instead of 24 hours) and found that 12-hour memory can occur in the absence of 24-hour long-term memory and that 12-hour memory does not rely on distributed training, protein synthesis or glutamate transmission. My results showed that both a massed training protocol and a distributed training protocol produce memory 12 hours following training. Memory 12 hours after massed training is mediated by a different mechanism than memory 12 hours after distributed training. For the purposes of this dissertation, memory types are considered distinct when they appear to rely on separate genes and/or mechanisms. Thus in this research, I have dissected apart at least three distinct forms of memory. Together these studies further our understanding of conditions required for memory to occur, and the mechanisms upon which memory relies.
Item Metadata
Title |
Behavioral analysis of memory in Caenorhabditis elegans
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Creator | |
Publisher |
University of British Columbia
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Date Issued |
2005
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Description |
Memory has been the focus of much research for more than 100 years. The earliest
documented memory experiments were published by Hermann Ebbinghaus in 1885. In
the current studies I investigated parameters affecting memory for habituation in the
nematode Caenorhabditis elegans and determined several cellular mechanisms that
underlie the behavioral expression of memory. I found that long-term memory for
habituation requires a distributed training protocol. I confirmed the generally accepted
view that mechanisms of memory consolidation occur after each training trial by showing
that protein synthesis inhibition delivered after every training trial effectively blocked
long-term memory. Glutamate has been postulated to play an important cellular role in
memory consolidation; the findings of these studies confirm that long-term memory for
habituation in C. elegans does not occur i f glutamate transmission is compromised. I
found that GLR-1 receptor activation is necessary for formation of long-term memory.
Further, using a GFP transgenic strain to measure GLR-1 expression, I observed a
significant decrease in GLR-1 24 hours after distributed training thus providing a putative
mechanism for memory. I examined the permanence of this glutamate dependent long-term
memory for habituation by inhibiting protein synthesis during memory
reconsolidation and found that this effectively erased the earlier consolidated memory.
Finally, I examined memory at an earlier time point (12 hours instead of 24 hours) and
found that 12-hour memory can occur in the absence of 24-hour long-term memory and
that 12-hour memory does not rely on distributed training, protein synthesis or glutamate
transmission. My results showed that both a massed training protocol and a distributed
training protocol produce memory 12 hours following training. Memory 12 hours after massed training is mediated by a different mechanism than memory 12 hours after
distributed training. For the purposes of this dissertation, memory types are considered
distinct when they appear to rely on separate genes and/or mechanisms. Thus in this
research, I have dissected apart at least three distinct forms of memory. Together these
studies further our understanding of conditions required for memory to occur, and the
mechanisms upon which memory relies.
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Genre | |
Type | |
Language |
eng
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Date Available |
2009-12-22
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Provider |
Vancouver : University of British Columbia Library
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Rights |
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
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DOI |
10.14288/1.0099809
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URI | |
Degree | |
Program | |
Affiliation | |
Degree Grantor |
University of British Columbia
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Graduation Date |
2005-11
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Campus | |
Scholarly Level |
Graduate
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Aggregated Source Repository |
DSpace
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Rights
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.