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Analysis of structual features of OprF from Pseudomonas aeruginosa using fusion proteins created by TnphoA mutagenesis Finnen, Renée Louise


The major outer membrane protein OprF from Pseudomonas aeruginosa was mutagenized with the specialized transposon TnphoA in order to investigate structural features of the protein. TnphoA insertions in the oprF gene were mapped by Sall digestion and determined to be located at 16 different sites across the gene. The precise location of at least one example of each insertion was determined by sequencing. In most cases the molecular weight of the OprF

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