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A novel T cell activating factor Williams, Laura Dawn
Abstract
The maturation of cytotoxic T lymphocyte (CTL) effectors from CTL precursors (CTLp) requires specific signals mediated through cellular interactions and soluble factors. The most studied factor is T cell growth factor (TCGF) which is also termed interleukin-2 (IL-2). This lymphokine is produced by T helper cells (TH) and induces activated CTLp to proliferate and differentiate. However, in the absence of mitogen or antigen stimulation, IL-2 alone cannot induce CTL (except in the case of very high cell density). A factor is described that is found in the supernatant of 4-β-phorbol-12-myristate-13-acetate (PMA)-induced EL4 cells that can polyclonally activate CTL in the presence of IL-2. This factor elutes at 27 kilodaltons (KDa) on a G-100 column, and its target cell includes T cells of the Thyl⁺ Lyt2⁺ L3T4⁻ phenotype. The factor increases the frequency of IL-2 receptor expressing cells within a population, thereby increasing the response to IL-2. It is suggested that this factor acts through an alternative pathway of CTL activation which is independent of specific stimulation by antigen.
Item Metadata
Title |
A novel T cell activating factor
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Creator | |
Publisher |
University of British Columbia
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Date Issued |
1987
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Description |
The maturation of cytotoxic T lymphocyte (CTL) effectors from CTL precursors (CTLp) requires specific signals mediated through cellular interactions and soluble factors. The most studied factor is T cell growth factor (TCGF) which is also termed interleukin-2 (IL-2). This lymphokine is produced by T helper cells (TH) and induces activated CTLp to proliferate and differentiate. However, in the absence of mitogen or antigen stimulation, IL-2 alone cannot induce CTL (except in the case of very high cell density). A factor is described that is found in the supernatant of 4-β-phorbol-12-myristate-13-acetate (PMA)-induced EL4 cells that can polyclonally activate CTL in the presence of IL-2. This factor elutes at 27 kilodaltons (KDa) on a G-100 column, and its target cell includes T cells of the Thyl⁺ Lyt2⁺ L3T4⁻ phenotype. The factor increases the frequency of IL-2 receptor expressing cells within a population, thereby increasing the response to IL-2. It is suggested that this factor acts through an alternative pathway of CTL activation which is independent of specific stimulation by antigen.
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Genre | |
Type | |
Language |
eng
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Date Available |
2010-07-19
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Provider |
Vancouver : University of British Columbia Library
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Rights |
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
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DOI |
10.14288/1.0097226
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URI | |
Degree | |
Program | |
Affiliation | |
Degree Grantor |
University of British Columbia
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Campus | |
Scholarly Level |
Graduate
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Aggregated Source Repository |
DSpace
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Item Media
Item Citations and Data
Rights
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.