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Isolation and characterization of DNA probes from the short arm of the human X chromosome which detect restriction fragment length polymorphisms Starr, Terence
Abstract
The isolation and characterization of DNA probes which detect RFLPs from a small region of the human genome, Xp, has been the focus of this thesis. Thirty X specific DNA probes were isolated from a flow sorted library constructed from a 49,XXXXY cell line. These probes were confirmed to be from the X chromosome by hybridization to dot blots containing DNA from mouse, human, and a somatic cell hybrid which contained the X chromosome as its only human DNA. Hybridization to somatic cell hybrids containing different regions of the X chromosome localized 9 of the probes to Xp. After testing 185 probe-enzyme combinations three of the Xp probes (localized to the distal 2/3 of Xp) were found to detect RFLPs. One probe, pTS316, was shown not to be inherited in a Mendelian fashion. The presence of one of the polymorphic bands detected by this probe correlated with the phenomenon of X chromosome inactivation.
Item Metadata
Title |
Isolation and characterization of DNA probes from the short arm of the human X chromosome which detect restriction fragment length polymorphisms
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Creator | |
Publisher |
University of British Columbia
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Date Issued |
1986
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Description |
The isolation and characterization of DNA probes which detect RFLPs from a small region of the human genome, Xp, has been the focus of this thesis. Thirty X specific DNA probes were isolated from a flow sorted library constructed from a 49,XXXXY cell line. These probes were confirmed to be from the X chromosome by hybridization to dot blots containing DNA from mouse, human, and a somatic cell hybrid which contained the X chromosome as its only human DNA. Hybridization to somatic cell hybrids containing different regions of the X chromosome localized 9 of the probes to Xp. After testing 185 probe-enzyme combinations three of the Xp probes (localized to the distal 2/3 of Xp) were found to detect RFLPs. One probe, pTS316, was shown not to be inherited in a Mendelian fashion. The presence of one of the polymorphic bands detected by this probe correlated with the phenomenon of X chromosome inactivation.
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Genre | |
Type | |
Language |
eng
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Date Available |
2010-06-30
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Provider |
Vancouver : University of British Columbia Library
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Rights |
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
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DOI |
10.14288/1.0096815
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URI | |
Degree | |
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Affiliation | |
Degree Grantor |
University of British Columbia
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Campus | |
Scholarly Level |
Graduate
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Aggregated Source Repository |
DSpace
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Rights
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.