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UBC Theses and Dissertations

Derivation of monoclonal antibodies to ferredoxin from Clostridium pasteurianum Weaver, Michael Stanley


Modifications to the standard techniques of somatic cell fusion are presented which have resulted in the derivation of hybrid cell lines secreting monoclonal antibody to the low molecular weight antigen ferredoxin. Antibody secreted by one of these lines has been purified using the technique of protein. A sepharose chromatography. Using standard immunochemical methods it has been determined that this monoclonal antibody has an approximate isoelectric pH of 8.5 - 8.6 and belongs to the IgG₂[sub b] subclass of immunoglobulin.

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