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Sequence analysis of transfer RNA 7 ser of Drosophila melanogaster Cribbs, David Lamar
Abstract
Sequence analysis of tRNA 7 ser from Drosophila melanogaster was
carried out, primarily by the formamide degradation and post-labeling method
of Stanley and Vassilenko. Preliminary analysis was on the terminal
nucleoside-5', 3'-bis [51 - ³²P] phosphates of electrophoretically separated
[51 - ³²P] ribooligonucleotides, identifying the [³² P]-nucleotides by
chromatography on PEI-cel1ulose plates. Further analysis of possible
modified nucleotides was performed by thin layer chromatography of [5' -³²P] nucleoside phosphates derived by nuclease P-j digestion from [51 -
³²P] oligomers. The partial sequence generated in this fashion was
supplemented by ladder gel sequence analysis of [51 - P]tRNA ser , and to a limited extent by two dimensional homochromatography. In this way, a sequence was obtained that is complete except for part of the aminoacyl stem and the 5'-end of the extra arm. The data are consistent with a sequence for tRNAser of pGCAGmUUGUGGCac4CGAGCGmGDDAAGGCXUCUGA— m3CUI GAi 6AAujCAGAUmUCCCUm3CUGGGAGm5CGUAGGTijjCGm1 AAUCCUACCGACUGCNCCA.
Item Metadata
| Title |
Sequence analysis of transfer RNA 7 ser of Drosophila melanogaster
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| Creator | |
| Publisher |
University of British Columbia
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| Date Issued |
1979
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| Description |
Sequence analysis of tRNA 7 ser from Drosophila melanogaster was
carried out, primarily by the formamide degradation and post-labeling method
of Stanley and Vassilenko. Preliminary analysis was on the terminal
nucleoside-5', 3'-bis [51 - ³²P] phosphates of electrophoretically separated
[51 - ³²P] ribooligonucleotides, identifying the [³² P]-nucleotides by
chromatography on PEI-cel1ulose plates. Further analysis of possible
modified nucleotides was performed by thin layer chromatography of [5' -³²P] nucleoside phosphates derived by nuclease P-j digestion from [51 -
³²P] oligomers. The partial sequence generated in this fashion was
supplemented by ladder gel sequence analysis of [51 - P]tRNA ser , and to a limited extent by two dimensional homochromatography. In this way, a sequence was obtained that is complete except for part of the aminoacyl stem and the 5'-end of the extra arm. The data are consistent with a sequence for tRNAser of pGCAGmUUGUGGCac4CGAGCGmGDDAAGGCXUCUGA— m3CUI GAi 6AAujCAGAUmUCCCUm3CUGGGAGm5CGUAGGTijjCGm1 AAUCCUACCGACUGCNCCA.
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| Genre | |
| Type | |
| Language |
eng
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| Date Available |
2010-03-05
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| Provider |
Vancouver : University of British Columbia Library
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| Rights |
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
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| DOI |
10.14288/1.0094654
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| URI | |
| Degree (Theses) | |
| Program (Theses) | |
| Affiliation | |
| Degree Grantor |
University of British Columbia
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| Campus | |
| Scholarly Level |
Graduate
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| Aggregated Source Repository |
DSpace
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Rights
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.