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Mechanism of delayed hypersensitivity reactions : in vitro and in vivo studies of the possible role of certain lymphokines in the development of delayed hypersensitivity reactions Wong, Fook Chuen


The aim of the present study was to determine how lymphokines could exert their biological action on the skin during the course of the delayed hypersensitivity reactions. Four groups of experiments were conducted to investigate: (1) The production and the separation of lymphokines, (2) the protease activity of lymphokines and the effect of lymphokines on the kinin-forming system, (3) the effect of lymphokines on mast cells and platelets, and (4) the effect of enzyme-treated lymphokines on the skin inflammatory reaction. Guinea pig lymph node lymphocytes, stimulated by either the specific antigen DNP-BGG or by concanavalin A, were used to generate lymphokines. Parameters for testing lymphokine activities were those of migration inhibitory factor (MIF) and skin reactive factor (SRF). Separation of MIF and SRF from the lymphokine preparation by gel filtration, electrophoresis and fractional precipitation with ammonium sulphate was unsuccessful, which indicated that the physical properties of MIF and SRF were similar. Lymphokine preparations contained little or no neutral and acidic protease activities.

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