- Library Home /
- Search Collections /
- Open Collections /
- Browse Collections /
- UBC Theses and Dissertations /
- Cytological alteration in the rat stomach postburn
Open Collections
UBC Theses and Dissertations
UBC Theses and Dissertations
Cytological alteration in the rat stomach postburn Harris, Curtis Norman
Abstract
Gastric mucosal erosions were induced in the glandular stomach of rats by scalding. The incorporation of Thymidine-methyl³-H into desoxyribonucleic acid was used to determine changes in gastric epithelial cell proliferating ability. Total desoxyribonucleic acid per milligram of gastric tissue was also determined. Sampling was done at twenty-four hours, seven days, and fifteen days postburn. Eighty-nine point two percent of rats with a standard 26.5 ± 2% scald burn had developed gastric mucosal erosions by twenty-four hours postburn. Seventeen point eight percent of burned rats had erosions by seven days and the incidence rose to 46.4% by fifteen days postburn. Ten point three percent of control rats in all sampling periods developed erosions. The total desoxyribonucleic acid in the gastric samples did not change significantly in any treatment period nor was it changed by treatment. Uptake of thymidine-methyl³-H was depressed twenty-four hours postburn and renewed so through seven days postburn. The results at the fifteen day sampling were inconclusive. By light microscope, the gastric surface epithelium was lifted from the lamina propria and at times there was complete denudation of this cell layer. The rat is a satisfactory animal model for gross study of mucosal erosions to at least fifteen day postburn. Because of eschar cannibalization inducing variable secretory status, the rat model was not suitable for thymidine uptake studies past seven days postburn.
Item Metadata
Title |
Cytological alteration in the rat stomach postburn
|
Creator | |
Publisher |
University of British Columbia
|
Date Issued |
1972
|
Description |
Gastric mucosal erosions were induced in the glandular stomach of rats by scalding. The incorporation of Thymidine-methyl³-H into desoxyribonucleic acid was used to determine changes in gastric epithelial cell proliferating ability. Total desoxyribonucleic acid per milligram of gastric tissue was also determined. Sampling was done at twenty-four hours, seven days, and fifteen days postburn.
Eighty-nine point two percent of rats with a standard 26.5 ± 2% scald burn had developed gastric mucosal erosions by twenty-four hours postburn. Seventeen point eight percent of burned rats had erosions by seven days and the incidence rose to 46.4% by fifteen days postburn. Ten point three percent of control rats in all sampling periods developed erosions.
The total desoxyribonucleic acid in the gastric samples did not change significantly in any treatment period nor was it changed by treatment. Uptake of thymidine-methyl³-H was depressed twenty-four hours postburn and renewed so through seven days postburn. The results at the fifteen day sampling were inconclusive.
By light microscope, the gastric surface epithelium was lifted from the lamina propria and at times there was complete denudation of this cell layer.
The rat is a satisfactory animal model for gross study of mucosal erosions to at least fifteen day postburn. Because of eschar cannibalization inducing variable secretory status, the rat model was not suitable for thymidine uptake studies past seven days postburn.
|
Genre | |
Type | |
Language |
eng
|
Date Available |
2011-04-28
|
Provider |
Vancouver : University of British Columbia Library
|
Rights |
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
|
DOI |
10.14288/1.0093273
|
URI | |
Degree | |
Program | |
Affiliation | |
Degree Grantor |
University of British Columbia
|
Campus | |
Scholarly Level |
Graduate
|
Aggregated Source Repository |
DSpace
|
Item Media
Item Citations and Data
Rights
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.