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An experimental investigation of autosomal translocations for insect pest control : fitness effects and marker-free isolation techniques Reid, John Arthur Keith

Abstract

Because of recent advances in genotic insect control theory, it has become important to investigate the fitness effects of, and isolation procedures for, homozygous autosomal translocations. I isolated 57 autosomal translocations in Drosophila melanoqaster. Of these 21 were homozygous viable. From data obtained during the isolation of these trunslocations and from fitness tests and competition cage experiments, rhe following points can be made; (1) Between one in ten and one in one hundred homozygous viable laboratory produced translocations are likely to be of value in field tests of genetic insect control procedures. (2) Translocations which produce high levels of unbalanced gametes when heterozygous do not tend to be lass fit in the homozygous state than others. Therefore screening procedures dependent only on reduced progeny production from translocation heterozygote parents should be satisfactory for the isolation of useable stocks. (3) Translocations whose breakpoints are vary near the center of chromosomes tend to produce small progeny reduction in the heterozygous state, making these translocations useless as negative heterotic carriers of useful genes. (4) Those translocations which are the result of multiple break events tend to be less fit than simple double-break translocations and therefore should be discarded.

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