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Expression of αvβ6 integrin in the junctional epithelium Garcia Fulle, Maria Isabel
Abstract
Cell-cell binding and cell-extracellular matrix adhesion is mediated by cell surface receptors known as integrins, which play important roles during wound healing. Junctional epithelium (JE) that links gingiva to tooth enamel mimics wound epithelium. The αvβ6 integrin is an epithelial integrin that is not normally expressed by oral gingival epithelium but is induced during wound healing, and has also been shown to be expressed in squamous cell carcinomas and in leukoplakia specimens. Furthermore, αvβ6 integrin can bind and activate transforming growth factor-β (TGF-β), suggesting a central role for the integrin in this unique epithelium. Using human gingival specimens from extracted teeth and human gingival specimens attached to decalcified teeth, the presence of JE was confirmed using simple epithelia tissue markers (cytokeratin-19 and laminin-5). The presence of αvβ6 integrin in the JE and some of its putative ligands (such as tenascin, fibronectin and TGF-β) was confirmed using immunohistochemical labelling. Paraffin sections of wild-type (FVB), β6integrin-overexpressing (B6F1) and P6integrin-knock-out mice (β6-/-) were stained with hematoxylin and eosin and observed under the light microscope to analyze the morphology of the JE. Interestingly, the analysis of the β6-/- sections showed striking alterations in the morphology and cellular organization of the JE. The findings of this study suggest that αvβ6 integrin is constitutively expressed in the JE, in which it could function as an immunoregulator via activation of TGF-β1.
Item Metadata
Title |
Expression of αvβ6 integrin in the junctional epithelium
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Creator | |
Publisher |
University of British Columbia
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Date Issued |
2005
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Description |
Cell-cell binding and cell-extracellular matrix adhesion is mediated by cell surface receptors
known as integrins, which play important roles during wound healing. Junctional epithelium (JE)
that links gingiva to tooth enamel mimics wound epithelium. The αvβ6 integrin is an epithelial
integrin that is not normally expressed by oral gingival epithelium but is induced during wound
healing, and has also been shown to be expressed in squamous cell carcinomas and in
leukoplakia specimens. Furthermore, αvβ6 integrin can bind and activate transforming growth
factor-β (TGF-β), suggesting a central role for the integrin in this unique epithelium. Using
human gingival specimens from extracted teeth and human gingival specimens attached to
decalcified teeth, the presence of JE was confirmed using simple epithelia tissue markers
(cytokeratin-19 and laminin-5). The presence of αvβ6 integrin in the JE and some of its putative
ligands (such as tenascin, fibronectin and TGF-β) was confirmed using immunohistochemical
labelling. Paraffin sections of wild-type (FVB), β6integrin-overexpressing (B6F1) and
P6integrin-knock-out mice (β6-/-) were stained with hematoxylin and eosin and observed under
the light microscope to analyze the morphology of the JE. Interestingly, the analysis of the β6-/-
sections showed striking alterations in the morphology and cellular organization of the JE. The
findings of this study suggest that αvβ6 integrin is constitutively expressed in the JE, in which it
could function as an immunoregulator via activation of TGF-β1.
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Genre | |
Type | |
Language |
eng
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Date Available |
2009-12-15
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Provider |
Vancouver : University of British Columbia Library
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Rights |
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
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DOI |
10.14288/1.0092184
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URI | |
Degree | |
Program | |
Affiliation | |
Degree Grantor |
University of British Columbia
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Graduation Date |
2005-11
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Campus | |
Scholarly Level |
Graduate
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Aggregated Source Repository |
DSpace
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Item Media
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Rights
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.