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Overexpression of NF-E2 related factor 2 via viral-mediated gene transfer in vivo. Imbeault, Sophie
Abstract
The transcription factor NF-E2 related factor 2 (Nrf2) is responsible for the upregulation of the cellular anti-oxidant defence system. Upon stimulation by free radicals or reactive oxygen species (ROS), the cytoplasmic-bound Nrf2 translocates to the nucleus and initiates transcription of genes containing anti-oxidant response elements (AREs) within their promoter region. Many ARE-containing genes contribute to the maintenance of redox homeostasis within the cell thus promoting cell survival. To date, limited work has been done looking at Nrf2-mediated neuroprotection in vivo. Here, we use viral-mediated gene delivery via stereotaxic injection into the adult Wistar rat brain in order to overexpress Nrf2. We exploit the capacity of 3-nitropropionic acid (3-NP) to produce ROS in order to provide an oxidative challenge to our animals. Histological analyses show reduced lesion size (lesions are ∼55 % smaller than controls, p
Item Metadata
Title |
Overexpression of NF-E2 related factor 2 via viral-mediated gene transfer in vivo.
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Creator | |
Publisher |
University of British Columbia
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Date Issued |
2005
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Description |
The transcription factor NF-E2 related factor 2 (Nrf2) is responsible for the upregulation of the cellular anti-oxidant defence system. Upon stimulation by free radicals or reactive oxygen species (ROS), the cytoplasmic-bound Nrf2 translocates to the nucleus and initiates transcription of genes containing anti-oxidant response elements (AREs) within their promoter region. Many ARE-containing genes contribute to the maintenance of redox homeostasis within the cell thus promoting cell survival. To date, limited work has been done looking at Nrf2-mediated neuroprotection in vivo. Here, we use viral-mediated gene delivery via stereotaxic injection into the adult Wistar rat brain in order to overexpress Nrf2. We exploit the capacity of 3-nitropropionic acid (3-NP) to produce ROS in order to provide an oxidative challenge to our animals. Histological analyses show reduced lesion size (lesions are ∼55 % smaller than controls, p
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Genre | |
Type | |
Language |
eng
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Date Available |
2009-12-11
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Provider |
Vancouver : University of British Columbia Library
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Rights |
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
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DOI |
10.14288/1.0092076
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URI | |
Degree (Theses) | |
Program (Theses) | |
Affiliation | |
Degree Grantor |
University of British Columbia
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Graduation Date |
2005-05
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Campus | |
Scholarly Level |
Graduate
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Aggregated Source Repository |
DSpace
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Rights
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.