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Fish analysis of chromosomal aneuploidies in spermatozoa from severely infertile men undergoing intracytoplasmic sperm injection (ICSI) Gao, Haijun
Abstract
BACKGROUND: The association between subsets of male infertility, oligoasthenoteratozoospermia (OAT), obstructive azoospermic (OA), non-obstructive azoospermia (NOA), and increased risk of having chromosomal abnormalities in the sperm has not been investigated. Chromosomal aneuploidy in sperm will be systematically investigated by standard methods with large sample sizes required to present significance. METHODS: Triple colour FISH analysis of chromosomes 18, X and Y and dual colour FISH analysis of chromosomes 13 and 21 were performed on spermatozoa retrieved from OAT patients, OA patients and NOA and control. RESULTS: 101,490 spermatozoa retrieved from OAT patients, 101,435 from OA patients, 12,200 from NOA patients and 101,486 from normal fertile men were examined. The three infertile patient groups had significantly increased rates of chromosomal aneuploidies (13.61% in OAT, 5.23% in OA, and 15.00% in NOA), compared with that in control group (1.50%; P< 0.0001). All the differences among the three infertile groups reached a significant level (P< 0.0001). The rates of nullisomy for sex chromosomes, and chromosomes 13,18 and 21 in three infertile groups were significantly higher than that in control group (P
Item Metadata
Title |
Fish analysis of chromosomal aneuploidies in spermatozoa from severely infertile men undergoing intracytoplasmic sperm injection (ICSI)
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Creator | |
Publisher |
University of British Columbia
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Date Issued |
2004
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Description |
BACKGROUND: The association between subsets of male infertility,
oligoasthenoteratozoospermia (OAT), obstructive azoospermic (OA), non-obstructive
azoospermia (NOA), and increased risk of having chromosomal abnormalities in the sperm has
not been investigated. Chromosomal aneuploidy in sperm will be systematically investigated by
standard methods with large sample sizes required to present significance.
METHODS: Triple colour FISH analysis of chromosomes 18, X and Y and dual colour FISH
analysis of chromosomes 13 and 21 were performed on spermatozoa retrieved from OAT
patients, OA patients and NOA and control.
RESULTS: 101,490 spermatozoa retrieved from OAT patients, 101,435 from OA patients,
12,200 from NOA patients and 101,486 from normal fertile men were examined. The three
infertile patient groups had significantly increased rates of chromosomal aneuploidies (13.61% in
OAT, 5.23% in OA, and 15.00% in NOA), compared with that in control group (1.50%; P<
0.0001). All the differences among the three infertile groups reached a significant level (P<
0.0001). The rates of nullisomy for sex chromosomes, and chromosomes 13,18 and 21 in three
infertile groups were significantly higher than that in control group (P
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Extent |
6997066 bytes
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Genre | |
Type | |
File Format |
application/pdf
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Language |
eng
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Date Available |
2009-12-02
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Provider |
Vancouver : University of British Columbia Library
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Rights |
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
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DOI |
10.14288/1.0091809
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URI | |
Degree (Theses) | |
Program (Theses) | |
Affiliation | |
Degree Grantor |
University of British Columbia
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Graduation Date |
2004-11
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Campus | |
Scholarly Level |
Graduate
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Aggregated Source Repository |
DSpace
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Item Media
Item Citations and Data
Rights
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.