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Individual bull variations on sperm acrosome reaction, sperm-zona binding, in-vitro embryo production, and preimplantation embryo apoptosis and gene expression Giritharan, Gnanaratnam

Abstract

The overall objective of this study was to develop in-vitro tests to predict fertility of bulls in the field. The specific objectives were to investigate 1) the bull effect on sperm acrosome reaction, sperm-zona binding and in vitro embryo production (experiment 1), 2) the effect of sperm pre-incubation time and sperm concentration of bulls on in-vitro fertilization (experiment 2), 3) the bull effect on apoptosis and expression of Bcl-2, Bax, p53, interferon tau and heat shock protein-70 genes in bovine preimplantation embryos produced in-vitro (experiment 3), and 4) the correlation between the above in-vitro tests and the field fertility data. In the first experiment, prefreeze motility, acrosome reaction at 0 h, increase in acrosome reaction at 4 h and sperm-zona binding were different (p

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