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UBC Theses and Dissertations
Interaction between NCX and SERPA in Ca²⁺ signaling in human endothelial cells Chan, Lally Lai Yee
Abstract
The interaction between sodium-calcium-exchanger (NCX) and the endoplasmic reticulum (ER) with respect to Ca²⁺ signaling was studied using fura-2 fluorescence imaging microscopy in human endothelial cells. The inflammatory agonist histamine was used to increase the intracellular Ca²⁺ concentration. Under resting conditions, the endothelial NCX serves to unload some of the Ca²⁺ content accumulated in the ER. This unloading is important in order to maintain the buffer barrier function of the peripheral ER. Application of histamine (1 uM) in the presence of extracellular Ca²⁺ caused a long lasting Ca²⁺ response. This maintained response is dependent on the state of ER Ca²⁺ content, which is at least partly refilled by Ca²⁺ entry via NCX working in the reverse mode during the course of agonist stimulation. After cessation of agonist stimulation, a major part of the increased Ca²⁺ is cleared by sarcoplasmic/endoplasmic reticulum Ca²⁺ - ATPase (SERCA) and NCX working in a serial configuration. In summary, in human endothelial cells, NCX can unload or refill the ER and thereby modulate the Ca²⁺ level.
Item Metadata
Title |
Interaction between NCX and SERPA in Ca²⁺ signaling in human endothelial cells
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Creator | |
Publisher |
University of British Columbia
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Date Issued |
2004
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Description |
The interaction between sodium-calcium-exchanger (NCX) and the endoplasmic
reticulum (ER) with respect to Ca²⁺ signaling was studied using fura-2
fluorescence imaging microscopy in human endothelial cells. The inflammatory
agonist histamine was used to increase the intracellular Ca²⁺ concentration.
Under resting conditions, the endothelial NCX serves to unload some of the Ca²⁺
content accumulated in the ER. This unloading is important in order to maintain
the buffer barrier function of the peripheral ER. Application of histamine (1 uM) in
the presence of extracellular Ca²⁺ caused a long lasting Ca²⁺ response. This
maintained response is dependent on the state of ER Ca²⁺ content, which is at
least partly refilled by Ca²⁺ entry via NCX working in the reverse mode during the
course of agonist stimulation. After cessation of agonist stimulation, a major part
of the increased Ca²⁺ is cleared by sarcoplasmic/endoplasmic reticulum Ca²⁺ -
ATPase (SERCA) and NCX working in a serial configuration. In summary, in
human endothelial cells, NCX can unload or refill the ER and thereby modulate
the Ca²⁺ level.
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Extent |
5376437 bytes
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Genre | |
Type | |
File Format |
application/pdf
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Language |
eng
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Date Available |
2009-11-21
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Provider |
Vancouver : University of British Columbia Library
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Rights |
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
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DOI |
10.14288/1.0091495
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URI | |
Degree | |
Program | |
Affiliation | |
Degree Grantor |
University of British Columbia
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Graduation Date |
2004-05
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Campus | |
Scholarly Level |
Graduate
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Aggregated Source Repository |
DSpace
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Item Media
Item Citations and Data
Rights
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.