- Library Home /
- Search Collections /
- Open Collections /
- Browse Collections /
- UBC Theses and Dissertations /
- Measurement of telomere organization using widefield...
Open Collections
UBC Theses and Dissertations
UBC Theses and Dissertations
Measurement of telomere organization using widefield microscopy Duncan, Tracy
Abstract
Widefield microscopy was used to study the location of telomeres in human interphase lymphocyte nuclei in order to determine whether telomeres are required for nuclear organization. To do this, a microscope's computer control system was programmed to automatically focus the microscope, and large numbers of images were analyzed. Cells in cell cycle stages G1 or S/G2 with long, medium, and short telomeres were photographed. Their two-dimensional images were analyzed to calculate the telomere distribution along the radius of the nucleus and to evaluate the degree of telomere clustering in space using Delaunay graphs. Telomeres in nuclei with short telomeres were shown to be distributed closer to the center of the nucleus than those in nuclei with long telomeres. The radial distribution of telomeres did not change with cell cycle phase. No changes in the degree of telomere clustering with telomere length or cell cycle phase were observed.
Item Metadata
Title |
Measurement of telomere organization using widefield microscopy
|
Creator | |
Publisher |
University of British Columbia
|
Date Issued |
2002
|
Description |
Widefield microscopy was used to study the location of telomeres in human interphase lymphocyte nuclei in order to determine whether telomeres are required for nuclear organization. To do this, a microscope's computer control system was programmed to automatically focus the microscope, and large numbers of images were analyzed. Cells in cell cycle stages G1 or S/G2 with long, medium, and short telomeres were photographed. Their two-dimensional images were analyzed to calculate the telomere distribution along the radius of the nucleus and to evaluate the degree of telomere clustering in space using Delaunay graphs. Telomeres in nuclei with short telomeres were shown to be distributed closer to the center of the nucleus than those in nuclei with long telomeres. The radial distribution of telomeres did not change with cell cycle phase. No changes in the degree of telomere clustering with telomere length or cell cycle phase were observed.
|
Extent |
4176762 bytes
|
Genre | |
Type | |
File Format |
application/pdf
|
Language |
eng
|
Date Available |
2009-10-17
|
Provider |
Vancouver : University of British Columbia Library
|
Rights |
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
|
DOI |
10.14288/1.0090973
|
URI | |
Degree | |
Program | |
Affiliation | |
Degree Grantor |
University of British Columbia
|
Graduation Date |
2002-05
|
Campus | |
Scholarly Level |
Graduate
|
Aggregated Source Repository |
DSpace
|
Item Media
Item Citations and Data
Rights
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.