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UBC Theses and Dissertations
Glutamine synthetase as a biological marker for fish phylogenetics : some new insights Laberge Macdonald, Tammy
Abstract
Glutamine synthetase is a key enzyme for nitrogen metabolism. It occurs in all organisms and is one of the oldest functioning genes. Many vertebrates have only one functional copy of this gene, while many plants have been shown to be multicopy for this gene. Pseudogenes for glutamine synthetase have also been reported in mammals. Until recently only a single copy of glutamine synthetase had been described in fish. However, six copies of this gene are expressed in rainbow trout Oncorhynchus myklss and two copies of this gene are expressed in the gulf toadfish Opsanus beta. We investigated a variety of intertidal fishes from British Columbia, Canada using PCR amplification of genomic DNA product and reverse transcriptase PCR to explore the diversity of glutamine synthetase in fish. We recovered two isoforms of glutamine synthetase in fourteen out of twenty-one fish. We describe the partial sequences for the two copies of this gene that differed in nucleotide composition by 8 to 22 percent. Phylogenetic analysis was performed using the different glutamine synthetase isoforms to generate trees for intertidal fishes collected in this study. Fish from the following orders were represented in this study: Myxiniformes, Lepisosteiformes, Salmoniformes, Gasterosteiformes, Syngnathiformes, Scorpaeniformes, Perciformes and Pleuronectiformes. Most species adhered to the traditional taxonomic classification although some representative fish did not.
Item Metadata
Title |
Glutamine synthetase as a biological marker for fish phylogenetics : some new insights
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Creator | |
Publisher |
University of British Columbia
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Date Issued |
2003
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Description |
Glutamine synthetase is a key enzyme for nitrogen metabolism. It occurs in all organisms and is one of the oldest functioning genes. Many vertebrates have only one functional copy of this gene, while many plants have been shown to be multicopy for this gene. Pseudogenes for glutamine synthetase have also been reported in mammals. Until recently only a single copy of glutamine synthetase had been described in fish. However, six copies of this gene are expressed in rainbow trout Oncorhynchus myklss and two copies of this gene are expressed in the gulf toadfish Opsanus beta. We investigated a variety of intertidal fishes from British Columbia, Canada using PCR amplification of genomic DNA product and reverse transcriptase PCR to explore the diversity of glutamine synthetase in fish. We recovered two isoforms of glutamine synthetase in fourteen out of twenty-one fish. We describe the partial sequences for the two copies of this gene that differed in nucleotide composition by 8 to 22 percent. Phylogenetic analysis was performed using the different glutamine synthetase isoforms to generate trees for intertidal fishes collected in this study. Fish from the following orders were represented in this study: Myxiniformes, Lepisosteiformes, Salmoniformes, Gasterosteiformes, Syngnathiformes, Scorpaeniformes, Perciformes and Pleuronectiformes. Most species adhered to the traditional taxonomic classification although some representative fish did not.
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Extent |
3244800 bytes
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Genre | |
Type | |
File Format |
application/pdf
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Language |
eng
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Date Available |
2009-10-17
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Provider |
Vancouver : University of British Columbia Library
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Rights |
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
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DOI |
10.14288/1.0090916
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URI | |
Degree | |
Program | |
Affiliation | |
Degree Grantor |
University of British Columbia
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Graduation Date |
2003-05
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Campus | |
Scholarly Level |
Graduate
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Aggregated Source Repository |
DSpace
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Item Media
Item Citations and Data
Rights
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.