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Cyclosporine A improves coronary artery function in rat cardiac allografts Moien-Afshari, Farzad


BACKGROUND: A marked decline in the vascular myogenic response during the course of allograft rejection was previously shown in a rat model of heart transplantation. Two important contributory features are an iNOS-catalyzed, NO-mediated vasodilation and a loss of smooth muscle function. In this study, the effect of cyclosporine A immunosuppressive therapy on the alleviation of myogenic and endothelial cell dysfunction of coronary resistance arteries in allograft was examined using pressure myography. We hypothesized that cyclosporine therapy will improve coronary artery function in rat cardiac allografts. METHODS: Coronary septal arteries (internal diameter 200μm) were dissected from heterotopic abdominal heart isografts (Lewis-Lewis) and allografts (Fisher-Lewis) at days 4 and 21 post-transplantation and mounted on a pressure myograph. Pressureinduced vasoconstriction was measured in the absence and presence of an iNOS blocker (aminoguanidine [AG], 100 μM). Endothelium based (ACh induced) and endothelium independent (SNP induced) vasorelaxation was also tested in each group and compared. RESULTS: Myogenic response was reduced in allograft coronary arteries at day 21 posttransplantation in comparison with matched isografts (p

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