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UBC Theses and Dissertations
The importance of cytoskeleton in neutrophil-cardiomyocyte interaction Davani, Ehsan Y.
Abstract
Activated polymorphonuclear neutrophils (PMN) contribute to decreased myocardial contractility after ischemia-reperfusion and in models of sepsis. To determine how PMN could contribute to myocardial dysfunction, rat ventricular cardiomyocytes were isolated and incubated with TNFα 20 ng/mL, IL-1β 20 ng/mL or LPS 10 pg/mL for 3 hours. Cardiomyocytes were then washed, co-cultured with isolated rat PMN for 2 hours, and electrically stimulated to determine fractional shortening (FS) using videomicroscopy. PMN co-cultured with activated cardiomyocytes reduced fractional shortening by 30±10% (p
Item Metadata
| Title |
The importance of cytoskeleton in neutrophil-cardiomyocyte interaction
|
| Creator | |
| Publisher |
University of British Columbia
|
| Date Issued |
2001
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| Description |
Activated polymorphonuclear neutrophils (PMN) contribute to decreased myocardial
contractility after ischemia-reperfusion and in models of sepsis. To determine how PMN
could contribute to myocardial dysfunction, rat ventricular cardiomyocytes were isolated
and incubated with TNFα 20 ng/mL, IL-1β 20 ng/mL or LPS 10 pg/mL for 3 hours.
Cardiomyocytes were then washed, co-cultured with isolated rat PMN for 2 hours, and
electrically stimulated to determine fractional shortening (FS) using videomicroscopy.
PMN co-cultured with activated cardiomyocytes reduced fractional shortening by 30±10%
(p
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| Extent |
7953219 bytes
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| Genre | |
| Type | |
| File Format |
application/pdf
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| Language |
eng
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| Date Available |
2009-08-12
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| Provider |
Vancouver : University of British Columbia Library
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| Rights |
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
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| DOI |
10.14288/1.0090199
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| URI | |
| Degree (Theses) | |
| Program (Theses) | |
| Affiliation | |
| Degree Grantor |
University of British Columbia
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| Graduation Date |
2002-05
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| Campus | |
| Scholarly Level |
Graduate
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| Aggregated Source Repository |
DSpace
|
Item Media
Item Citations and Data
Rights
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.