UBC Theses and Dissertations
The importance of cytoskeleton in neutrophil-cardiomyocyte interaction Davani, Ehsan Y.
Activated polymorphonuclear neutrophils (PMN) contribute to decreased myocardial contractility after ischemia-reperfusion and in models of sepsis. To determine how PMN could contribute to myocardial dysfunction, rat ventricular cardiomyocytes were isolated and incubated with TNFα 20 ng/mL, IL-1β 20 ng/mL or LPS 10 pg/mL for 3 hours. Cardiomyocytes were then washed, co-cultured with isolated rat PMN for 2 hours, and electrically stimulated to determine fractional shortening (FS) using videomicroscopy. PMN co-cultured with activated cardiomyocytes reduced fractional shortening by 30±10% (p
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