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Magnesium status and peroxidative stress in Golden Syrian hamsters fed casein and soy based diets

University of British Columbia

The overall objective of this thesis was to utilize casein and soy based diets containing different levels of magnesium and/or phytate to show the effect of magnesium status and bioavailability on the susceptibility to lipid peroxidation in hamsters. Magnesium deficiency has been shown to enhance oxidative stress in experimental animals. Since cholesterol metabolism in hamsters closely resembles that of humans, in contrast to other rodent species, the Golden-Syrian hamster lipid model, under casein and soy fed magnesium sufficient and magnesium deficient diets, was used to test the hypothesis that magnesium deficiency may enhance oxidative stress. In experiment 1, 32 male Golden-Syrian Hamsters (n = 8) were randomly assigned to magnesium-depleted (185 ± 3 ppm) and magnesium-repleted (653 ± 4 ppm) diets alongside supplemented phytate (0.5%) and non-supplemented phytate (0%) diets. Diets in experiment 1 were casein based. After 4 weeks on the experimental diets, hamster magnesium status, as shown by magnesium concentrations in the kidney and heart, was mainly affected by dietary magnesium (p<0.05) in hamsters not by supplemented dietary phytate. Oxidative stress was evaluated in terms of levels of thiobarbituric acid-reactive substances (TBARS) in the liver as well as modified ApoB levels in Hamster LDL during time dependent forced peroxidation with hydrogen peroxide (H2O2). Both dietary magnesium and dietary supplemented phytate were determining factors in the hamster's ability to cope with oxidative stress (p<0.05). In experiment 2, 32 male Golden-Syrian Hamsters (n = 8) were randomly assigned to magnesium-depleted (191 ± 2 ppm) and magnesium-repleted (761 ± 4 ppm) diets alongside supplemented phytate (0.5%) and non- supplemented phytate (0%) diets. Diets in experiment 2 were soy based. After 4 weeks on the experimental diets, hamster magnesium status was mainly affected by dietary magnesium (p<0.05) in hamsters not by supplemented dietary phytate. Both dietary magnesium and dietary supplemented phytate were determining factors in the hamster's ability to cope with oxidative stress (p<0.05).

Thesis/Dissertation

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2009-08-06

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http://hdl.handle.net/2429/11793

Food Science

University of British Columbia

UBCV

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