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The effects of genetic manipulation on the stress response and disease resistance of coho salmon Jhingan, Esther
Abstract
Genetic manipulation is widely used for the improvement of agricultural organisms. However, alterations of the genome could also result in unintentional changes, and could perhaps alter the performance of the organism. Performance traits such as sensitivity to stressors and to pathogens are strongly influenced by genetic information, and could therefore be affected by alterations of the genome. In this thesis, I tested the hypothesis that genetic manipulation alters the stress response and disease resistance of fish. Specifically, the studies examined the effects of the insertion of a growth hormone (GH) gene construct and triploidy on coho salmon (Oncorhynchus kisutch). In this study, the GH transgene did not alter the physiological or cellular stress responses to a sudden heat shock, relative to those of non-transgenic fish. Exposure of the diploid fish to a bacterial pathogen (Vibrio anguillarum) appeared to reveal differences in disease resistance between different transgenic lines. The first filial generation of GH transgenic fish, which were also a more rapidly growing line, appeared to be more susceptible to disease relative to non-transgenic fish. The second filial generation of GH transgenic fish, which were a slower growing transgenic line, were equally or more resistant than the non-transgenic fish. Disease resistance was compromised by triploidy, and presence of the GH transgene in triploid fish did not appear to further modify the triploid's resistance to the pathogen.
Item Metadata
Title |
The effects of genetic manipulation on the stress response and disease resistance of coho salmon
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Creator | |
Publisher |
University of British Columbia
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Date Issued |
1998
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Description |
Genetic manipulation is widely used for the improvement of agricultural organisms.
However, alterations of the genome could also result in unintentional changes, and
could perhaps alter the performance of the organism. Performance traits such as
sensitivity to stressors and to pathogens are strongly influenced by genetic information,
and could therefore be affected by alterations of the genome. In this thesis, I tested the
hypothesis that genetic manipulation alters the stress response and disease resistance
of fish. Specifically, the studies examined the effects of the insertion of a growth
hormone (GH) gene construct and triploidy on coho salmon (Oncorhynchus kisutch).
In this study, the GH transgene did not alter the physiological or cellular stress
responses to a sudden heat shock, relative to those of non-transgenic fish. Exposure of
the diploid fish to a bacterial pathogen (Vibrio anguillarum) appeared to reveal
differences in disease resistance between different transgenic lines. The first filial
generation of GH transgenic fish, which were also a more rapidly growing line,
appeared to be more susceptible to disease relative to non-transgenic fish. The second
filial generation of GH transgenic fish, which were a slower growing transgenic line,
were equally or more resistant than the non-transgenic fish. Disease resistance was
compromised by triploidy, and presence of the GH transgene in triploid fish did not
appear to further modify the triploid's resistance to the pathogen.
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Extent |
3846805 bytes
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Genre | |
Type | |
File Format |
application/pdf
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Language |
eng
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Date Available |
2009-06-11
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Provider |
Vancouver : University of British Columbia Library
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Rights |
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
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DOI |
10.14288/1.0088904
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URI | |
Degree | |
Program | |
Affiliation | |
Degree Grantor |
University of British Columbia
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Graduation Date |
1999-05
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Campus | |
Scholarly Level |
Graduate
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Aggregated Source Repository |
DSpace
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Item Media
Item Citations and Data
Rights
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.