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UBC Theses and Dissertations
Influence of diet fat saturation on rates of cholesterol synthesis and esterification in healthy young men Mazier, Marie Jeanne Patricia
Abstract
To examine the effect of diet fat type on rates of cholesterol synthesis and esterification during feeding and fasting, nine healthy male subjects were fed solid-food diets of 40% fat as predominantly either olive oil (MONO), safflower-oil margarine (POLY), or butter (SAT). At the end of each two-week diet trial, subjects were given deuterium (D) oxide orally and de novo synthesis was measured from D incorporation into cholesterol and interpreted as rates of fractional synthesis (FSR) (pools/day) into the rapidly exchangeable free cholesterol (FC) pool. Absolute synthesis rates (ASR) were calculated as the product of FSR and the FC pool. Pool size for each subject was obtained from analysis of the specific activity decay curve of an intravenous injection of 4-14C-cholesterol over nine months. Synthesis was measured over two consecutive 12-h fed periods followed by two consecutive 12-h fasted periods. Serum samples were also assayed for lathosterol concentration, an index of cholesterol synthesis. Serum cholesterol and non-HDL cholesterol concentrations were highest on the SAT diet, lowest (P
Item Metadata
Title |
Influence of diet fat saturation on rates of cholesterol synthesis and esterification in healthy young men
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Creator | |
Publisher |
University of British Columbia
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Date Issued |
1994
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Description |
To examine the effect of diet fat type on rates of cholesterol
synthesis and esterification during feeding and fasting, nine healthy
male subjects were fed solid-food diets of 40% fat as predominantly
either olive oil (MONO), safflower-oil margarine (POLY), or butter
(SAT). At the end of each two-week diet trial, subjects were given
deuterium (D) oxide orally and de novo synthesis was measured from D
incorporation into cholesterol and interpreted as rates of fractional
synthesis (FSR) (pools/day) into the rapidly exchangeable free
cholesterol (FC) pool. Absolute synthesis rates (ASR) were calculated
as the product of FSR and the FC pool. Pool size for each subject was
obtained from analysis of the specific activity decay curve of an
intravenous injection of 4-14C-cholesterol over nine months. Synthesis
was measured over two consecutive 12-h fed periods followed by two
consecutive 12-h fasted periods. Serum samples were also assayed for
lathosterol concentration, an index of cholesterol synthesis. Serum
cholesterol and non-HDL cholesterol concentrations were highest on the
SAT diet, lowest (P
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Extent |
13498376 bytes
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Genre | |
Type | |
File Format |
application/pdf
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Language |
eng
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Date Available |
2009-06-09
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Provider |
Vancouver : University of British Columbia Library
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Rights |
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
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DOI |
10.14288/1.0088878
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URI | |
Degree (Theses) | |
Program (Theses) | |
Affiliation | |
Degree Grantor |
University of British Columbia
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Graduation Date |
1995-05
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Campus | |
Scholarly Level |
Graduate
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Aggregated Source Repository |
DSpace
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Item Media
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Rights
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.