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Characterization of classical pathway complement activation by liposomes and modulation by incorporated poly (ethylene glycol) Bradley, Amanda Joan
Abstract
Complement activation causes opsonization of foreign particles leading to particle elimination from the blood. Complement-mediated opsonization of charged and large liposomes presents a fundamental problem in their use to deliver therapeutic agents in vivo. To prolong the circulation half-lives of such liposomes, complement activation must be curtailed. The two overall aims of this study were to characterize complement activation through antibody-independent C1q binding to anionic liposomes and to assess the ability of poly(ethylene glycol)-lipids (PEG-lipids) to inhibit this complement activation. This study determined that both electrostatic and chemical forces contributed to C1q binding to anionic liposomes. Negative phospholipids in the liposome composition were required to detect C1 q binding to liposomes. At close to physiologic pH (7.2) and ionic strength (0.145 M) and in the absence or presence of serum, anionic liposomes bound a measurable but small amount of C1q . However, as the concentration of negative phospholipid in the liposomes increased, C1q binding increased. C1q binding increased even more as the ionic strength or the pH decreased. C1q peptide studies also demonstrated the importance of electrostatics. Peptides composed of residues 14-26 of the C1qA chain (C1qA₁₄[sub -]₂₆ ) bearing a net charge of plus five inhibited C1q binding and complement activation by anionic liposomes. This inhibitory capacity was dependent on the peptide's five positive charges and was independent of conformation or amino acidsequence. While electrostatics were important in determining C1q saturation binding, C1q binding affinity constants were independent of the electrostatic component suggesting that chemical forces were also involved. Clq-mediated complement activation was also affected by liposome size. For cardiolipin-containing liposomes, 240 nm vesicles bound more C1q and activated complement more readily than 100 nm vesicles. Multilamellar vesicles (∽1-8 μm) bound 15 times more C1q than 240 nm liposomes. This study is the first to show the ability of PEG-lipids to act as a barrier against complement activation by anionic liposomes. Incorporation of either cholesterol-PEG₆₀₀ (CH-PEG₆₀₀) , cholesterol-PEG₁₀₀₀ (CH-PEG₁₀₀₀) , or phosphatidylethanolamine-PEG₂₀₀₀ (PE-PEG₂₀₀₀) caused PEG-lipid dose-dependent inhibition of C1q binding to and complement activation by large anionic liposomes. Complement activation was strongly inhibited when 15 mole % of CH-PEG₆₀₀, 10 mole % CH - PEG₁₀₀₀, or 5 mole % PE - PEG₂₀₀₀ were incorporated into 100 nm anionic liposomes.
Item Metadata
Title |
Characterization of classical pathway complement activation by liposomes and modulation by incorporated poly (ethylene glycol)
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Creator | |
Publisher |
University of British Columbia
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Date Issued |
1998
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Description |
Complement activation causes opsonization of foreign particles leading to particle
elimination from the blood. Complement-mediated opsonization of charged and large
liposomes presents a fundamental problem in their use to deliver therapeutic agents in
vivo. To prolong the circulation half-lives of such liposomes, complement activation must
be curtailed. The two overall aims of this study were to characterize complement
activation through antibody-independent C1q binding to anionic liposomes and to assess
the ability of poly(ethylene glycol)-lipids (PEG-lipids) to inhibit this complement
activation.
This study determined that both electrostatic and chemical forces contributed to
C1q binding to anionic liposomes. Negative phospholipids in the liposome composition
were required to detect C1 q binding to liposomes. At close to physiologic pH (7.2) and
ionic strength (0.145 M) and in the absence or presence of serum, anionic liposomes
bound a measurable but small amount of C1q . However, as the concentration of negative
phospholipid in the liposomes increased, C1q binding increased. C1q binding increased
even more as the ionic strength or the pH decreased. C1q peptide studies also
demonstrated the importance of electrostatics. Peptides composed of residues 14-26 of the
C1qA chain (C1qA₁₄[sub -]₂₆ ) bearing a net charge of plus five inhibited C1q binding and
complement activation by anionic liposomes. This inhibitory capacity was dependent on
the peptide's five positive charges and was independent of conformation or amino acidsequence.
While electrostatics were important in determining C1q saturation binding, C1q
binding affinity constants were independent of the electrostatic component suggesting that chemical forces were also involved.
Clq-mediated complement activation was also affected by liposome size. For
cardiolipin-containing liposomes, 240 nm vesicles bound more C1q and activated
complement more readily than 100 nm vesicles. Multilamellar vesicles (∽1-8 μm) bound 15
times more C1q than 240 nm liposomes.
This study is the first to show the ability of PEG-lipids to act as a barrier against
complement activation by anionic liposomes. Incorporation of either cholesterol-PEG₆₀₀
(CH-PEG₆₀₀) , cholesterol-PEG₁₀₀₀ (CH-PEG₁₀₀₀) , or phosphatidylethanolamine-PEG₂₀₀₀
(PE-PEG₂₀₀₀) caused PEG-lipid dose-dependent inhibition of C1q binding to and
complement activation by large anionic liposomes. Complement activation was strongly
inhibited when 15 mole % of CH-PEG₆₀₀, 10 mole % CH - PEG₁₀₀₀, or 5 mole % PE -
PEG₂₀₀₀ were incorporated into 100 nm anionic liposomes.
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Extent |
9886956 bytes
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Genre | |
Type | |
File Format |
application/pdf
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Language |
eng
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Date Available |
2009-06-02
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Provider |
Vancouver : University of British Columbia Library
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Rights |
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
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DOI |
10.14288/1.0088736
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URI | |
Degree | |
Program | |
Affiliation | |
Degree Grantor |
University of British Columbia
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Graduation Date |
1998-05
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Campus | |
Scholarly Level |
Graduate
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Aggregated Source Repository |
DSpace
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Item Media
Item Citations and Data
Rights
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.