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UBC Theses and Dissertations
Quantification of the tissue changes in the human lung with chronic lung disease using a combination of computed tomography and stereology Coxson, Harvey Owen
Abstract
Idiopathic pulmonary fibrosis (IPF) and pulmonary emphysema are chronic lung diseases exhibiting progressive deterioration in pulmonary function as the lung architecture is remodeled. This thesis quantifies these tissue changes using a novel combination of computed tomography (CT) and quantitative histology. Pre-operative CT scans were obtained from patients with IPF, patients receiving lung volume reduction surgery for diffuse emphysema and from patients with minimal to mild emphysema undergoing lobectomy for a small peripheral tumour. Total lung volume was calculated using the pixel dimensions on the CT scan while airspace and tissue volume as well as the regional lung expansion were estimated using the attenuation values. Tissue samples were obtained at either open lung biopsy (IPF) or surgical resection (control and emphysema) and prepared for quantitative histology. A method for correcting the histology specimens to an in vivo level of inflation was developed so that tissue composition and surface area could be estimated using stereologic techniques. The data shows that there is a reorganization of lung parenchyma in IPF with a disproportionate of airspace and surface area without increasing the total amount of tissue. The patients with emphysema show evidence of a progressive proteolytic destruction of tissue volume and surface area. There is a negative correlation between regional lung expansion and surface area in emphysema and a positive correlation between surface area and the diffusing capacity of the lung in both diseases. This technique should prove useful in the longitudinal assessment of chronic lung diseases and the monitoring of response to treatment.
Item Metadata
Title |
Quantification of the tissue changes in the human lung with chronic lung disease using a combination of computed tomography and stereology
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Creator | |
Publisher |
University of British Columbia
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Date Issued |
1998
|
Description |
Idiopathic pulmonary fibrosis (IPF) and pulmonary emphysema are chronic lung
diseases exhibiting progressive deterioration in pulmonary function as the lung architecture is
remodeled. This thesis quantifies these tissue changes using a novel combination of computed
tomography (CT) and quantitative histology. Pre-operative CT scans were obtained from
patients with IPF, patients receiving lung volume reduction surgery for diffuse emphysema and
from patients with minimal to mild emphysema undergoing lobectomy for a small peripheral tumour. Total lung volume was calculated using the pixel dimensions on the CT scan while
airspace and tissue volume as well as the regional lung expansion were estimated using the attenuation values. Tissue samples were obtained at either open lung biopsy (IPF) or
surgical resection (control and emphysema) and prepared for quantitative histology. A method
for correcting the histology specimens to an in vivo level of inflation was developed so that tissue composition and surface area could be estimated using stereologic techniques. The
data shows that there is a reorganization of lung parenchyma in IPF with a disproportionate of airspace and surface area without increasing the total amount of tissue. The patients with
emphysema show evidence of a progressive proteolytic destruction of tissue volume and
surface area. There is a negative correlation between regional lung expansion and surface
area in emphysema and a positive correlation between surface area and the diffusing capacity
of the lung in both diseases. This technique should prove useful in the longitudinal assessment
of chronic lung diseases and the monitoring of response to treatment.
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Extent |
11425632 bytes
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Genre | |
Type | |
File Format |
application/pdf
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Language |
eng
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Date Available |
2009-05-29
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Provider |
Vancouver : University of British Columbia Library
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Rights |
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
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DOI |
10.14288/1.0088702
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URI | |
Degree | |
Program | |
Affiliation | |
Degree Grantor |
University of British Columbia
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Graduation Date |
1998-05
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Campus | |
Scholarly Level |
Graduate
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Aggregated Source Repository |
DSpace
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Item Media
Item Citations and Data
Rights
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.