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The development of the Pseudomonas aeruginosa outer membrane protein OprF as a presentation vector for foreign antigenic determinants

University of British Columbia

A variety of systems have been developed to improve the presentation of foreign antigenic determinants (‘epitopes’) by inserting them in the context of carrier proteins. The goals of this study were to develop the Pseudomonas aeruginosa outer membrane protein OprF as a carrier for foreign epitopes and to study the effect of the mode of presentation on the antigenicity of the presented epitope. The model epitope used in this study was the 4-amino acid repeating epitope (NANP) of the circumsporozoite protein of the malaria parasite, Plasmodium falciparum. Linker-insertion mutagenesis was carried out to create 11 “permissive” sites which allowed the insertion of 4 extra amino acids. Two series of OprF

Thesis/Dissertation

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2009-04-22

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http://hdl.handle.net/2429/7463

Microbiology and Immunology

University of British Columbia

UBCV

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