- Library Home /
- Search Collections /
- Open Collections /
- Browse Collections /
- UBC Theses and Dissertations /
- Expression of keratinocyte integrins in acute and chronic...
Open Collections
UBC Theses and Dissertations
UBC Theses and Dissertations
Expression of keratinocyte integrins in acute and chronic inflammation of the human periodontium Larjava, Kirsi Haapasalmi
Abstract
Epithelium plays a crucial role in protection, destruction and repair of human mucosal and epidermal tissues. Integrins compose a family of heterodimeric transmembrane glycoproteins that mediate cell adhesion and information transfer from extracellular milieu into the cytoplasm. In the present work, the expression of integrins in human mucosal and epidermal wound keratinocytes during acute (wound healing) and chronic inflammation (periodontitis) was investigated. Integrins and their ligands were localized in frozen sections using immunohistochemistry. During wound healing, expression of avB6 integrin was induced relatively late during wound healing. Maximal expression was observed in 7-day-old wounds, in which epithelial sheets had joined and granulation tissue was present. Fibronectin and tenascin, both possible ligands for avP36 integrin, were concentrated underneath the basal keratinocytes expressing this integrin. During chronic inflammation, focal loss of integrins and basement membrane components was common in periodontal pocket epithelium. In some local areas, however, upregulation of integrin expression was observed. The results suggest, that during acute inflammation, the reservoir of the keratinocyte integrins increases to correspond to new demands for keratinocyte migration and gene regulation. During chronic inflammation, however, the expression of integrins in keratinocytes is variable, suggesting focal areas of integrin up- and down-regulation that may possibly reflect the areas of healing and destruction in the periodontium.
Item Metadata
Title |
Expression of keratinocyte integrins in acute and chronic inflammation of the human periodontium
|
Creator | |
Publisher |
University of British Columbia
|
Date Issued |
1997
|
Description |
Epithelium plays a crucial role in protection, destruction and repair of human mucosal
and epidermal tissues. Integrins compose a family of heterodimeric transmembrane
glycoproteins that mediate cell adhesion and information transfer from extracellular
milieu into the cytoplasm. In the present work, the expression of integrins in human
mucosal and epidermal wound keratinocytes during acute (wound healing) and chronic
inflammation (periodontitis) was investigated. Integrins and their ligands were
localized in frozen sections using immunohistochemistry. During wound healing,
expression of avB6 integrin was induced relatively late during wound healing. Maximal
expression was observed in 7-day-old wounds, in which epithelial sheets had joined and
granulation tissue was present. Fibronectin and tenascin, both possible ligands for
avP36 integrin, were concentrated underneath the basal keratinocytes expressing this
integrin. During chronic inflammation, focal loss of integrins and basement membrane
components was common in periodontal pocket epithelium. In some local areas,
however, upregulation of integrin expression was observed. The results suggest, that
during acute inflammation, the reservoir of the keratinocyte integrins increases to
correspond to new demands for keratinocyte migration and gene regulation. During
chronic inflammation, however, the expression of integrins in keratinocytes is variable,
suggesting focal areas of integrin up- and down-regulation that may possibly reflect
the areas of healing and destruction in the periodontium.
|
Extent |
11027669 bytes
|
Genre | |
Type | |
File Format |
application/pdf
|
Language |
eng
|
Date Available |
2009-03-25
|
Provider |
Vancouver : University of British Columbia Library
|
Rights |
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
|
DOI |
10.14288/1.0087968
|
URI | |
Degree | |
Program | |
Affiliation | |
Degree Grantor |
University of British Columbia
|
Graduation Date |
1997-11
|
Campus | |
Scholarly Level |
Graduate
|
Aggregated Source Repository |
DSpace
|
Item Media
Item Citations and Data
Rights
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.