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Phospocreatine resynthesis after exercise and hypoxia in the carp (cyprinus carpio) : a 31P-NMRS study Hallman, Troy M.
Abstract
Phosphocreatine (PCr) was reduced in carp muscle by exercise or hypoxia at 15°C and 25°C in order to assess the influence of pH and temperature in PCr synthesis in vivo. Intramuscular PCr levels and intracellular pH (pH,) values were determined by 31P-NMRS. Exercise resulted in a significantly more acidic pHi than following hypoxia, differing by 0.38 and 0.44 pH units at 15°C and 25°C, respectively. The lower pH values in exercised carp slowed the recovery of PCr compared with posthypoxic fish, requiring a four-fold increase in the amount of time necessary to rebuild PCr levels. Throughout the recovery period. PCr levels following hypoxia were consistently and significantly higher than those following exercise. Intracellular pH continued to fall for up to over two hours, dropping to less than 6.3 and 6.7 following exercise and hypoxia, respectively. This continuing decline in pHi was correlated with the rise in PCr levels up to the time when PCr was nearly recovered. During hypoxia, 25°C acclimated carp depleted PCr stores more quickly than those at 15°C, temperature had no effect on the time for recovery of either PCr or pHi.
Item Metadata
Title |
Phospocreatine resynthesis after exercise and hypoxia in the carp (cyprinus carpio) : a 31P-NMRS study
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Creator | |
Publisher |
University of British Columbia
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Date Issued |
1997
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Description |
Phosphocreatine (PCr) was reduced in carp muscle by exercise or hypoxia at 15°C
and 25°C in order to assess the influence of pH and temperature in PCr synthesis in
vivo. Intramuscular PCr levels and intracellular pH (pH,) values were determined
by 31P-NMRS. Exercise resulted in a significantly more acidic pHi than following
hypoxia, differing by 0.38 and 0.44 pH units at 15°C and 25°C, respectively. The
lower pH values in exercised carp slowed the recovery of PCr compared with posthypoxic
fish, requiring a four-fold increase in the amount of time necessary to
rebuild PCr levels. Throughout the recovery period. PCr levels following hypoxia
were consistently and significantly higher than those following exercise.
Intracellular pH continued to fall for up to over two hours, dropping to less than 6.3
and 6.7 following exercise and hypoxia, respectively. This continuing decline in pHi
was correlated with the rise in PCr levels up to the time when PCr was nearly
recovered. During hypoxia, 25°C acclimated carp depleted PCr stores more quickly
than those at 15°C, temperature had no effect on the time for recovery of either PCr
or pHi.
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Extent |
3556369 bytes
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Genre | |
Type | |
File Format |
application/pdf
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Language |
eng
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Date Available |
2009-03-25
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Provider |
Vancouver : University of British Columbia Library
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Rights |
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
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DOI |
10.14288/1.0087965
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URI | |
Degree | |
Program | |
Affiliation | |
Degree Grantor |
University of British Columbia
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Graduation Date |
1997-11
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Campus | |
Scholarly Level |
Graduate
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Aggregated Source Repository |
DSpace
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Item Media
Item Citations and Data
Rights
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.